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Sandra Martha Gomes Dias Lab: HuR (ELAVL1) silencing sensitizes triple-negative breast cancer to glutaminase-targeted therapy
Sandra Martha Gomes Dias
Unpublished

Plasmids from Article

ID Plasmid Purpose  
110386pQC V5 HuR.wt IRES Puroγ-Retroviral transfer vector for expressing HuR (wild type), IRES-driven Puromycin selection.
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110387pQC V5 mKO2 HuR.wt IRES Puroγ-Retroviral transfer vector for expressing HuR (wild type), V5 and mKO2 tags, IRES-driven Puromycin selection.
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110389pQC V5 mKO2 HuR.DelHNS IRES Puroγ-Retroviral transfer vector for expressing HuR (delHNS), V5 and mKO2 tags, IRES-driven Puromycin selection.
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110390pQC hKGA.wt V5-His IRES G418γ-Retroviral transfer vector for expressing glutminase isoform KGA (wild type), C-terminal V5-6xHis tags, IRES-driven Geneticin selection.
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110391pQC hKGA.S286A V5-His IRES G418γ-Retroviral transfer vector for expressing glutminase isoform KGA (S286A), C-terminal V5-6xHis tags, IRES-driven Geneticin selection.
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110401psiCHECK2 3'UTR GACLuminescence-based glutaminase GAC isoform 3'UTR-mediated RNA stability reporter
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110402psiCHECK2 3'UTR KGALuminescence-based glutaminase KGA isoform 3'UTR-mediated RNA stability reporter
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110403pX330.puroCRISPR/Cas9 vector with sgRNA expression and puromycin resistance
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110404pX330.orupCRISPR/Cas9 vector with sgRNA expression and puromycin resistance
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110405pX330.puro sgRNA KGA Stop1CRISPR/Cas9 vector with sgRNA for glutaminase KGA isoform C-Terminal Knock-in and puromycin resistance
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110406pUC19 KGA HA5'-mKO2-3'Homology donor for glutaminase KGA isoform knock-in, mKO2
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110408pUC19 KGA HA5'-mKO2-P2A-Zeo-3'Homology donor for glutaminase KGA isoform knock-in, mKO2-P2A-Zeo
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110409pLKO.1-blast shLucshLuc (Target TTACGCTGAGTACTTCGA), silence LUC gene, blasticidin selection.
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110410pLKO.1-blast shGACshGAC (Target CCTCTGTTCTGTCAGAGTT), silence glutaminase isoform GAC, blasticidin selection.
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110411tet-pLKO.puro_shHuR CDSTRCN0000276129 (Target CGAGCTCAGAGGTGATCAAAG), silence human ELAVL1 (HuR) gene, doxycycline inducible, puromycin selection
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110412tet-pLKO.puro_shHuR 3UTRTRCN0000276186 (Target TTGTTAGTGTACAACTCATTT), silence human ELAVL1 (HuR) gene, doxycycline inducible, puromycin selection
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110414pLKO.puro_shHuR 3UTRTRCN0000276186 (Target TTGTTAGTGTACAACTCATTT), silence human ELAVL1 (HuR) gene, puromycin selection
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Antibodies from Article