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The Human Knockout Gene CLYBL Connects Itaconate to Vitamin B12.
Shen H, Campanello GC, Flicker D, Grabarek Z, Hu J, Luo C, Banerjee R, Mootha VK
Cell. 2017 Nov 2;171(4):771-782.e11. doi: 10.1016/j.cell.2017.09.051. Epub 2017 Oct 19. PubMed Article

Plasmids from Article

ID Plasmid Purpose
111288HsCLYBL(aa23-340)-Hisbacterial expression
111289HsCLYBL(aa23-340) D320N-Hisbacterial expression
111290HsCLYBL-FLAGmammalian expression
111291HsCLYBL(D320A)-FLAGmammalian expression
111292CaMCLC-Hisbacterial expression
111293His-PaIctbacterial expression
111294MsCLYBL_sgRNA1CRISPR KO murine CLYBL
111295MsCLYBL_sgRNA6CRISPR KO murine CLYBL
111296MsMUT_sgRNA1CRISPR KO murine MUT
111297MsMUT_sgRNA3CRISPR KO murine MUT
111298Neg. Ctrl_sgRNACRISPR KO murine neg. ctrl.

Antibodies from Article