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StaPLs: versatile genetically encoded modules for engineering drug-inducible proteins.
Jacobs CL, Badiee RK, Lin MZ
Nat Methods. 2018 Jul;15(7):523-526. doi: 10.1038/s41592-018-0041-z. Epub 2018 Jul 2. PubMed Article

Plasmids from Article

ID Plasmid Purpose
111500pCS6-YFP-SMASh(AI)Expresses Venus YFP with a C-terminal SMASh(AI) tag fusion in mammalian cells. [AI = asunaprevir inhibited].
111501pCS6-YFP-SMASh(TI)Expresses Venus YFP with a C-terminal SMASh(TI) tag fusion in mammalian cells. [TI = telaprevir inhibited].
111502pCS6-ZF(VEGFA)-StaPL(AI)-YFP-VPRExpresses a zinc finger specific to the human VEGFA locus, which is linked to a VPR transcriptional activation domain via a StaPL(AI) module, in mammalian cells. [AI = asunaprevir inhibited].
111504pCS6-ZF(VEGFA)-StaPL(TI)-tdRFP-KRABExpresses a zinc finger specific to the human VEGFA locus, which is linked to a KRAB transcriptional repression domain via a StaPL(TI) module, in mammalian cells. [TI = telaprevir inhibited].
111508pPB-VPR-dSpCas9[StaPL(TI)]Expresses a VPR transcriptional activator fused to the nuclease-deactivated S. pyogenes Cas9, which is governed by an inserted StaPL(TI) module, in mammalian cells. [TI = telaprevir inhibited].
111511pCS6-StaPLd-Casp9Expresses a tandem caspase-9 dimer, governed by a StaPL(AI) module, in mammalian cells. [AI = asunaprevir inhibited].

Antibodies from Article