Design and characterization of mutant and wildtype huntingtin proteins produced from a toolkit of scalable eukaryotic expression systems.
Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith CH
J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 10.1074/jbc.RA118.007204. Epub 2019 Mar 6. PubMed Article
Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith CH
J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 10.1074/jbc.RA118.007204. Epub 2019 Mar 6. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 111723 | pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q23 | Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells |
| 111724 | pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q73 | Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells |
| 111725 | pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q79 | Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells |
| 111726 | pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q23 | Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells |
| 111727 | pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q54 | Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells |
| 111728 | pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q73 | Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells |
| 111729 | pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q79 | Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells |
| 111730 | pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q109 | Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells |
| 111731 | pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q145 | Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells |
| 111732 | pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q15 | Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells |
| 111733 | pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q19 | Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells |
| 111734 | pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q51 | Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells |
| 111735 | pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q66 | Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells |
| 111736 | pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q85 | Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells |
| 111737 | pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q78 | Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells |
| 111738 | pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q139 | Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells |
| 111741 | pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q19 | Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells |
| 111742 | pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q24 | Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells |
| 111743 | pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q25 | Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells |
| 111744 | pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q30 | Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells |
| 111745 | pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q36 | Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells |
| 111746 | pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q42 | Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells |
| 111747 | pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q48 | Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells |
| 111748 | pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q52 | Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells |
| 111749 | pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q60 | Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells |
| 111750 | pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q66 | Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells |
| 111751 | pBacMam2-DiEx-LIC-N entry vector | Intermediate vector containing HTT gene, except for polyQ region. Used to clone various polyQ lengths into HTT sequence with N-term FLAG tag. Baculovirus transfer vector for insect and mammalian cells |
| 111752 | pBacMam2-DiEx-LIC-C entry vector | Intermediate vector containing HTT gene, except for polyQ region. Used to clone various polyQ lengths into HTT sequence with C-term FLAG tag. Baculovirus transfer vector for insect and mammalian cells |
| 124060 | HAP40 1-371 | Baculovirus expression vector for HAP40 protein (aa 1-371) in insect cells |