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Structural architecture of a dimeric class C GPCR based on co-trafficking of sweet taste receptor subunits.
Park J, Selvam B, Sanematsu K, Shigemura N, Shukla D, Procko E
J Biol Chem. 2019 Feb 5. pii: RA118.006173. doi: 10.1074/jbc.RA118.006173. PubMed Article

Plasmids from Article

ID Plasmid Purpose
113944pcDNA3.1(+)-HA-Flag-natT1R2mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin
113945pCEP4-HA-Flag-natT1R2mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin
113946pcDNA3.1(+)-HLA-cmyc-natT1R2mammalian expression plasmid for c-myc-tagged human T1R2 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain
113947pcDNA3.1(+)-HLA-Flag-natT1R2mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain
113948pCEP4-HLA-cmyc-optiT1R3 a21-852mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain
113949pcDNA3.1(+)-HLA-cmyc-optiT1R3 a21-852mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain
113950pcDNA3.1(+)-HLA-Flag-natT1R3mammalian expression plasmid for FLAG-tagged human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain
113951pCEP4-HA-Flag-natT1R2 a22-end_S212Nmammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant
113952pCEP4-HA-Flag-natT1R2 a22-end_I132S-S212Nmammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant
113953pCEP4-HA-Flag-natT1R2 a22-end_I132S-S212N-D231Gmammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant
113954pCEP4-HA-Flag-natT1R2 ECDmammalian expression plasmid for FLAG-tagged human T1R2 extracellular domain with signal peptide of influenza hemagglutinin
113955pCEP4-HA-Flag-natT1R2 FS1mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant
113956pCEP4-HA-Flag-natT1R2 FS10mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant
113957pCEP4-HA-Flag-natT1R2 ECD-MHCmammalian expression plasmid for FLAG-tagged human T1R2 ECD with signal peptide of influenza hemagglutinin fused to a canonical transmembrane domain from MHC class I
113958pCEP4-HA-Flag-natT1R2 RR837-8GGmammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant
113959pCEP4-HA-Flag-natT1R2 d835mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant
113960pCEP4-HLA-c-myc-optiT1R3 ECDmammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 extracellular domain with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain
113961pCEP4-HLA-c-myc-optiT1R3 ECD-MHCmammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 extracellular domain with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain fused to a canonical
113962pcDNA3-HLA-cMyc-EcopT1R1mammalian expression plasmid for c-myc-tagged E. coli codon-optimized human T1R1 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

Antibodies from Article