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A Bacterial Expression Vector Archive (BEVA) for Flexible Modular Assembly of Golden Gate-Compatible Vectors.
Geddes BA, Mendoza-Suarez MA, Poole PS
Front Microbiol. 2019 Jan 14;9:3345. doi: 10.3389/fmicb.2018.03345. eCollection 2018.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
113978pOGG001Level 0 module. nptII promoter for constitutive gene expression
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113979pOGG004Vector construction module. Level 1 golden gate cloning site module with LacZ reporter
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113980pOGG005Level 1 high copy number vector for E. coli expression
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113981pOGG006Golden Gate Level 2 cloning site, position 1 module for vector construction
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113982pOGG008Vector construction module. Kanamycin/neomycin resistance module for construction of Golden Gate cloning vectors
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113983pOGG009Vector construction module. Gentamicin resistance module for construction of Golden Gate cloning vectors
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113984pOGG010Vector construction module. RK2 (IncP) low-copy number origin of replication for construction of Golden Gate cloning vectors
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113985pOGG011Vector construction module. pBBR1 medium-copy number origin of replication for construction of Golden Gate cloning vectors
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113986pOGG012Vector construction module. Partition genes (parABCDE) for plasmid stability in absence of antibiotic selection for construction of Golden Gate cloning vectors
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113987pOGG013Vector construction module. Endlinker/terminator module to circularize plasmid following Part 3
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113988pOGG014Vector construction module. Endlinker/terminator module to circularize plasmid following Part 4
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113989pOGG015Vector construction module. Endlinker/terminator module to circularize plasmid following Part 5
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113990pOGG016Vector construction module. Endlinker/terminator module to circularize plasmid following Part 6
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113991pOGG024Level 1 golden gate cloning vector for medium copy number free-living expression
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113992pOGG026Level 1 golden gate cloning vector for low copy number stable environmental gene expression
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113993pOGG030Level 0 module. IPTG-inducible T7RNAP promoter for BL21 protein purification
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113994pOGG031Level 0 module. IPTG promoter for inducible expression
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113995pOGG037SC module. Super-folder GFP green fluorescence reporter
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113996pOGG039SC module. Terminator for T7RNAP
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113997pOGG041Level 0 module. Taurine-inducible promoter for Alphaproteobacteria
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113998pOGG042Vector construction module. Tetracycline resistance module for construction of Golden Gate cloning vectors
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113999pOGG050SC module. Thermostable beta-galactosidase reporter
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114000pOGG082Level 0 module. NifH promoter for nodule-specific expression
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114001pOGG083SC module. Beta-glucosidase reporter
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114002pOGG216Level 2 golden gate cloning vector for medium copy number free-living expression
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115503pOGG202Clone of a level 1 unit which encodes IPTG-inducible sfGFP
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115504pOGG203Clone of a level 1 unit which encodes a constitutively-expressed mCherry
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115505pOPS0253Suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing gusA.
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115506pOPS0254Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing celB
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115507pOPS0314Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing celB
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115508pOPS0359 Reporter Plasmid. Taurine-inducible expression of GFP
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115509pOPS0377Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing sfGFP
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115510pOPS0379Suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing sfGFP
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115511pOPS0750Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing gusA.
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Antibodies from Article