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Discovery of widespread Type I and Type V CRISPR-Cas inhibitors.
Marino ND, Zhang JY, Borges AL, Sousa AA, Leon LM, Rauch BJ, Walton RT, Berry JD, Joung JK, Kleinstiver BP, Bondy-Denomy J
Science. 2018 Sep 6. pii: science.aau5174. doi: 10.1126/science.aau5174.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
115136pCMV-T7-hAcrVA1-NLS(sv40) (BPK5050)CMV-T7 promoter expression plasmid for human codon optimized AcrVA1 anti-CRISPR protein with C-terminal NLS
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115137pCMV-T7-hAcrVA2.1-NLS(sv40) (BPK5059)CMV-T7 promoter expression plasmid for human codon optimized AcrVA2.1 anti-CRISPR protein with C-terminal NLS
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115138pCMV-T7-hAcrVA2-NLS(sv40) (AAS2283)CMV-T7 promoter expression plasmid for human codon optimized AcrVA2 anti-CRISPR protein with C-terminal NLS
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115139pCMV-T7-hAcrVA3.1-NLS(sv40) (RTW2624)CMV-T7 promoter expression plasmid for human codon optimized AcrVA3.1 anti-CRISPR protein with C-terminal NLS
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115140pCMV-T7-hAcrVA3-NLS(sv40) (BPK5077)CMV-T7 promoter expression plasmid for human codon optimized AcrVA3 anti-CRISPR protein with C-terminal NLS
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115141pCMV-T7-hOrf2mor-NLS(sv40) (BPK5095)CMV-T7 promoter expression plasmid for human codon optimized Orf2mor anti-CRISPR protein with C-terminal NLS
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115142pCAG-hMb3Cas12a-NLS(nucleoplasmin)-3xHA (RTW2500)CAG promoter expression plasmid for human codon optimized Mb3Cas12a nuclease with C-terminal NLS and HA tag
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Antibodies from Article