New Human Chromosomal Sites with "Safe Harbor" Potential for Targeted Transgene Insertion.
Pellenz S, Phelps M, Tang W, Hovde BT, Sinit RB, Fu W, Li H, Chen E, Monnat RJ Jr
Hum Gene Ther. 2019 Jul;30(7):814-828. doi: 10.1089/hum.2018.169. Epub 2019 Mar 28. PubMed Article
Pellenz S, Phelps M, Tang W, Hovde BT, Sinit RB, Fu W, Li H, Chen E, Monnat RJ Jr
Hum Gene Ther. 2019 Jul;30(7):814-828. doi: 10.1089/hum.2018.169. Epub 2019 Mar 28. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 115143 | pSH231-EF1-Puro | Safe harbor site 231 knock-in vector with puromycin resistance selection cassette |
| 115144 | pSH231-EF1-GFP-HYGRO | Safe harbor site 231 knock-in vector with GFP-HygromycinR expression cassette |
| 115145 | pSH231-EF1-RFP-HYGRO | Safe harbor site 231 knock-in vector with RFP-HygromycinR expression cassette |
| 115146 | pSH231-EFS-Cas9-BlastR | Safe harbor site 231 knock-in vector with Cas9-BlastR expression cassette |
| 115147 | pSH231-EF1-BLST-Cas9-VPR | Safe harbor site 231 knock-in vector with BlastR-Cas9-VPR expression cassette |
| 115148 | pSH231-EF1-BLST-dCas9-VPR | Safe harbor site 231 knock-in vector with BlastR-dCas9-VPR expression cassette |
| 115149 | pSH231-Bx-GFP-C31 | Empty safe harbor site 231 knock-in vector with flanking homology arms and Bxb1 and C31 attP integrase landing pads |
| 115150 | pUS2-SH231 | Cas9-GFP, dual gRNA expression vector for targeted integration of repair templates into safe harbor 231 locus in chromosome 4 |