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Addgene
Calcium stabilizes the strongest protein fold.
Milles LF, Unterauer EM, Nicolaus T, Gaub HE
Nat Commun. 2018 Nov 12;9(1):4764. doi: 10.1038/s41467-018-07145-6. PubMed Article

Plasmids from Article

ID Plasmid Purpose
117979pET28a-SdrG_N2N3-B1-B2-HIS-ybbrSdrG N2 and N3, B1 and B2 domains with C-terminal ybbr tag for covalent immobilization
117980pET28a-SdrG_N2N3-B1-HIS-ybbrSdrG N2 and N3, B1 domains with C-terminal ybbr tag for covalent immobilization
117981pET28a-MGGG-ybbr-HIS-SdrG_B1-DKSdrG B1 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfB
117982pET28a-MGGG-ybbr-HIS-SdrG_B2-DKSdrG B2 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfB
117983pET28a-MGGG-ybbr-HIS-SdrD_B1-DKSdrD B1 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfB

Antibodies from Article