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Examining multiple cellular pathways at once using multiplex hextuple luciferase assaying.
Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young DW, Venken KJT
Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
118043pUPD3GoldenBraid2.0 Universal Part Domesticator #3
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118044Alpha1GoldenBraid2.0 Alpha 1 destination vector
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118045Alpha2GoldenBraid2.0 Alpha 2 destination vector
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118046Omega 1GoldenBraid2.0 Omega 1 destination vector
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118047Omega 2GoldenBraid2.0 Omega 2 destination vector
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118048phCMV-IE1GBPart - Cytomegalovirus enhancer and promoter for constitutive high level expression in mammalian cells
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118049pMiniPGBPart - Minimal Promoter for expression in mammalian cells
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118050p5xNF-kb_REGBPart - Operator/DNA Response element - 5 copies of the NF-kb DNA binding motif
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118051p4xSMAD_REGBPart - Operator/DNA Response element - 4 copies the SMAD DNA binding motif
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118052p5xE-BoxGBPart - Operator/DNA Response element - 5 copies of the E-box motif
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118053p2xp53_REGBPart - Operator/DNA Response element - 2 copies of the p53 DNA binding motif
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118054p6xAP-1_REGBPart - Operator/DNA Response element - 6 copies of the AP-1 binding motif
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118056pELucGBPart - ELuc Luciferase CDS
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118057pRedFGBPart - RedF Luciferase CDS
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118058pNLucGBPart - NLuc Luciferase CDS
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118059pRenillaGBPart - Renilla Luciferase CDS
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118060pGrRenillaGBPart - GreenRenilla Luciferase CDS
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118061pbGHGBPart - Bovine growth hormone terminator
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118062hCMV-IE1:ELucTranscriptional unit for constitutive expression of ELuc in mammalian cells
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118063hCMV-IE1:FLucTranscriptional unit for constitutive expression of FLuc in mammalian cells
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118064hCMV-IE1:RedFTranscriptional unit for constitutive expression of RedF in mammalian cells
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118065hCMV-IE1:NLucTranscriptional unit for constitutive expression of NLuc in mammalian cells
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118066hCMV-IE1:RenillaTranscriptional unit for constitutive expression of Renilla in mammalian cells
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118067hCMV-IE1:GrRenillaTranscriptional unit for constitutive expression of GrRenilla in mammalian cells
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118068p(A)n-PAUSETranscription Blocker - Synthetic polyadenylation signal & RNA polymerase II transcriptional pause signal from the human α2 globin gene
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118069MLRVMulti-luciferase reporter vector including transcriptional reporters for NF-kb, TGF-b, c-Myc, p53 and MAPK/JNK transcriptional reporters
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135283pCGBGBPart - CGB Luciferase CDS
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135284pRoLucGBPart - RoLuc Luciferase CDS
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135286pMetLucGBPart - MetLuc Luciferase CDS
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135287pLuciaGBPart - Lucia Luciferase CDS
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135288pGLucGBPart - GLuc Luciferase CDS
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135290hCMV-IE1:RoLuc:bgHTranscriptional unit for constitutive expression of RoLuc in mammalian cells
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135292hCMV-IE1:MetLuc:bgHTranscriptional unit for constitutive expression of MetLuc in mammalian cells
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135293hCMV-IE1:Lucia:bgHTranscriptional unit for constitutive expression of Lucia luciferase in mammalian cells
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135294hCMV-IE1:GLuc:bgHTranscriptional unit for constitutive expression of GLuc in mammalian cells
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Antibodies from Article