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OTUB1 non-catalytically stabilizes the E2 ubiquitin-conjugating enzyme UBE2E1 by preventing its autoubiquitination.
Pasupala N, Morrow ME, Que LT, Malynn BA, Ma A, Wolberger C
J Biol Chem. 2018 Nov 23;293(47):18285-18295. doi: 10.1074/jbc.RA118.004677. Epub 2018 Oct 3. PubMed Article

Plasmids from Article

ID Plasmid Purpose
118209FLAG-OTUB1 WT-pcDNA3.1Expresses FLAG-OTUB1 WT in mammalian cells
118210FLAG-OTUB1 C91S-pcDNA3.1Expresses FLAG-OTUB1 C91S catalytic inactive mutant in mammalian cells
118211FLAG-OTUB1 T134R-pcDNA3.1Expresses FLAG-OTUB1 T134R mutant in mammalian cells
118212FLAG-OTUB1 T134R,C91S-pcDNA3.1Expresses FLAG-OTUB1 T134R,C91S in mammalian cells
118213HA-UBE2E1 WT in pcDNA3.1Expresses HA-UBE2E1 in mammalian cells
118214HA-UBE2E1 C131A in pcDNA3.1Expresses HA-UBE2E1 C131A catalytic mutant in mammalian cells

Antibodies from Article