Coordinated histone modifications and chromatin reorganization in a single cell revealed by FRET biosensors.
Peng Q, Lu S, Shi Y, Pan Y, Limsakul P, Chernov AV, Qiu J, Chai X, Shi Y, Wang P, Ji Y, Li YJ, Strongin AY, Verkhusha VV, Izpisua Belmonte JC, Ren B, Wang Y, Chien S, Wang Y
Proc Natl Acad Sci U S A. 2018 Nov 26. pii: 1811818115. doi: 10.1073/pnas.1811818115. PubMed Article
Peng Q, Lu S, Shi Y, Pan Y, Limsakul P, Chernov AV, Qiu J, Chai X, Shi Y, Wang P, Ji Y, Li YJ, Strongin AY, Verkhusha VV, Izpisua Belmonte JC, Ren B, Wang Y, Chien S, Wang Y
Proc Natl Acad Sci U S A. 2018 Nov 26. pii: 1811818115. doi: 10.1073/pnas.1811818115. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 120802 | H3K9me3 biosensor | FRET biosensor. To monitor histone H3 lysine 9 tri-methylation in mammalian cells by FRET |
| 120807 | K9L mutant of H3K9me3 biosensor | FRET biosensor. Eliminating the H3 lysine 9 methylation site in H3K9me3 biosensor to abolish the detection capability |
| 120808 | W45A mutant of H3K9me3 biosensor | FRET biosensor. Disrupting HP1 binding capability in H3K9me3 biosensor to abolish the detection capability |
| 120809 | H3S10ph biosensor | FRET biosensor. To monitor histone H3 Serine 10 phosphorylation in mammalian cells by FRET |
| 120810 | S10A mutant of H3S10ph biosensor | FRET biosensor. Eliminating the H3 Serine 10 phosphorylation site in H3S10ph biosensor to abolish the detection capability |