Continuous evolution of base editors with expanded target compatibility and improved activity.
Thuronyi BW, Koblan LW, Levy JM, Yeh WH, Zheng C, Newby GA, Wilson C, Bhaumik M, Shubina-Oleinik O, Holt JR, Liu DR
Nat Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 10.1038/s41587-019-0193-0. PubMed Article
Thuronyi BW, Koblan LW, Levy JM, Yeh WH, Zheng C, Newby GA, Wilson C, Bhaumik M, Shubina-Oleinik O, Holt JR, Liu DR
Nat Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 10.1038/s41587-019-0193-0. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 122595 | pBT2dR3-proB | BE PACE selection, express T7 RNA polymerase with degron-GTCCA target |
| 122596 | pBT13-splitC | clone M13 phage by SapI Golden Gate |
| 122597 | pBT13-splitD | clone M13 phage by SapI Golden Gate |
| 122598 | pBT29-splitC | clone M13 phage by SapI Golden Gate |
| 122599 | pBT29-splitD | clone M13 phage by SapI Golden Gate |
| 122600 | pBT44c | BE PACE selection, express C-intein-Cas9-ugi fusion |
| 122601 | pBT100.106b | clone APOBEC1 phage by SapI Golden Gate |
| 122602 | pBT100.110 | clone CDA1 phage by SapI Golden Gate |
| 122603 | pBT100.136c | clone FERNY phage by SapI Golden Gate |
| 122604 | pBT120b | BE PACE selection, express gIII, luxAB and degron-GTCCA guide RNA |
| 122605 | pBT137-splitC | clone M13 phage by SapI Golden Gate |
| 122606 | pBT137-splitD | clone M13 phage by SapI Golden Gate |
| 122607 | pBT276 | express CDA1-BE4max in mammalian cells |
| 122608 | pBT277 | express evoCDA1-BE4max in mammalian cells |
| 122609 | pBT279 | express FERNY-BE4max in mammalian cells |
| 122610 | pBT280 | express evoFERNY-BE4max in mammalian cells |
| 122611 | pBT281 | express evoAPOBEC1-BE4max in mammalian cells |
| 125612 | pBT371 | Expresses CDA1-BE4max-NG in mammalian cells |
| 125613 | pBT372 | Expresses evoCDA1-BE4max-NG in mammalian cells |
| 125614 | pBT373 | Expresses FERNY-BE4max-NG in mammalian cells |
| 125615 | pBT374 | Expresses evoFERNY-BE4max-NG in mammalian cells |
| 125616 | pBT375 | Expresses evoAPOBEC1-BE4max-NG in mammalian cells |
| 125617 | pBT376 | Expresses BE4max-NG in mammalian cells |
| 127843 | pBT195 | clone BE4max CBEs by SapI Golden Gate |
| 127844 | pBT362 | clone BE4max-NG CBEs by SapI Golden Gate |
| 127845 | pBT271.359 | clone A3A CBEs by SapI Golden Gate |
| 127846 | pBT271.361 | clone A3A-W98Y CBEs by SapI Golden Gate |