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Probing the modularity of megasynthases by rational engineering of a fatty acid synthase Type I.
Rittner A, Paithankar KS, Drexler DJ, Himmler A, Grininger M
Protein Sci. 2019 Feb;28(2):414-428. doi: 10.1002/pro.3550. Epub 2018 Dec 20.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
122846pAR17_StrepI_hFASh_H8_pET22bExpresses human type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.
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122847pAR18_StrepI_mFASm_H8_pET22bExpresses murine type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.
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122849pAR69_STRI_m(KS_MAT)_H8_pET22bExpresses the condensing part of murine type I fatty acid synthase (FASN) consisting of the KS and MAT domain in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.
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122850pAR127_StrepI_m(KS_MAT)_mouseL_ACP_H8_pET22bExpresses a designed construct of the condensing part fused to the ACP domain of murine type I fatty acid synthase (FASN) in Escherichia coli
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122851pAR357_SFP_pCDF-1bCo-expresses the phosphopantheteine transferase Sfp of Bacillus subtilis to transfer a PPant arm to acyl carrier proteins in Escherichia coli. Untagged; in pCDF-1b vector.
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Antibodies from Article