Enhanced mammalian genome editing by new Cas12a orthologs with optimized crRNA scaffolds.
Teng F, Li J, Cui T, Xu K, Guo L, Gao Q, Feng G, Chen C, Han D, Zhou Q, Li W
Genome Biol. 2019 Feb 5;20(1):15. doi: 10.1186/s13059-019-1620-8. PubMed Article
Teng F, Li J, Cui T, Xu K, Guo L, Gao Q, Feng G, Chen C, Han D, Zhou Q, Li W
Genome Biol. 2019 Feb 5;20(1):15. doi: 10.1186/s13059-019-1620-8. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 123632 | pCAG-ArCas12a-2AeGFP | Mammalian expression, Genome editing |
| 123633 | pCAG-BsCas12a-2AeGFP | Mammalian expression, Genome editing |
| 123634 | pCAG-HkCas12a-2AeGFP | Mammalian expression, Genome editing |
| 123635 | pCAG-LpCas12a-2AeGFP | Mammalian expression, Genome editing |
| 123636 | pCAG-PrCas12a-2AeGFP | Mammalian expression, Genome editing |
| 123637 | pCAG-PxCas12a-2AeGFP | Mammalian expression, Genome editing |
| 123638 | pCAG-AsCas12a-2AeGFP | Mammalian expression, Genome editing |
| 123639 | pUC19-U6-crRNA1 | Mammalian expression, Genome editing, gRNA scaffold |
| 123640 | pUC19-U6-crRNA4n96 | Mammalian expression, Genome editing, gRNA scaffold |
| 123641 | pUC19-U6-tRNAx2 | Mammalian expression, Genome editing, backbone |