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Generating Single Cell-Derived Knockout Clones in Mammalian Cells with CRISPR/Cas9.
Giuliano CJ, Lin A, Girish V, Sheltzer JM
Curr Protoc Mol Biol. 2019 Sep;128(1):e100. doi: 10.1002/cpmb.100.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
120577LRG2.1-BFPLentiviral plasmid to co-express a guide RNA and EBFP.
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124770Lenti-Cas9-gRNA-GFP3rd generation lentiviral plasmid encoding Cas9, GFP, and a gRNA backbone
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124771LRG2.1-CyOFP13rd generation lentiviral plasmid to co-express a guide RNA and CyOFP1
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124772LRG2.1-mOrange3rd generation lentiviral plasmid to co-express a guide RNA and mOrange
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124773LRG2.1-TagBFP2Lentiviral plasmid to co-express a guide RNA and TagBFP2
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124774Lenti-Cas9-gRNA-TagBFP23rd generation lentiviral plasmid encoding Cas9, TagBFP2, and a gRNA backbone
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Antibodies from Article