Crystal structure of cis-aconitate decarboxylase reveals the impact of naturally occurring human mutations on itaconate synthesis.
Chen F, Lukat P, Iqbal AA, Saile K, Kaever V, van den Heuvel J, Blankenfeldt W, Bussow K, Pessler F
Proc Natl Acad Sci U S A. 2019 Sep 23. pii: 1908770116. doi: 10.1073/pnas.1908770116. PubMed Article
Chen F, Lukat P, Iqbal AA, Saile K, Kaever V, van den Heuvel J, Blankenfeldt W, Bussow K, Pessler F
Proc Natl Acad Sci U S A. 2019 Sep 23. pii: 1908770116. doi: 10.1073/pnas.1908770116. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 124842 | pCAD16_CAD_12-490_pvp008 | Expresses Aspergillus terreus CAD in E.coli |
| 124843 | pCAD29_hIRG1_4-461_pvp008 | Expresses human CAD (Irg1) in E.coli |
| 124873 | pCAD39_mIRG1_4-462_pvp008 | Expresses mouse CAD (Irg1) in E.coli |
| 124874 | pCAD96_hIRG1_4-461_pvp008 | Expresses hCAD Asn152Ser mutant in E.coli |
| 124875 | pCAD100_hIRG1_4-461_pvp008 | Expresses hCAD Arg273His mutant in E.coli |
| 124876 | pCAD54_CAD29_H104A_pvp008 | Expresses hCAD His103Ala mutant in E.coli |
| 124877 | pCAD104_Asn152Ser_pCMV6Entry-hIRG1 | Expresses hCAD Asp152Ser mutant in mammalian cells |
| 124878 | pCAD108_Arg273His_pCMV6Entry-hIRG1 | Expresses hCAD Arg273His mutant in mammalian cells |
| 124879 | pCAD64_pCMV6_Entry_hIrg1 | Expresses hCAD His103Ala mutant in mammalian cells |