Luciferase-LOV BRET enables versatile and specific transcriptional readout of cellular protein-protein interactions.
Kim CK, Cho KF, Kim MW, Ting AY
Elife. 2019 Apr 3;8. pii: 43826. doi: 10.7554/eLife.43826. PubMed Article
Kim CK, Cho KF, Kim MW, Ting AY
Elife. 2019 Apr 3;8. pii: 43826. doi: 10.7554/eLife.43826. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 125224 | pAAV-HA-β2AR-NNES-NanoLuc-15aa linker-AsLOV2(V416L) | Expresses β2AR-NanoLuc-AsLOV2(V416L) in mammalian cells |
| 125225 | pAAV-gLuc sp-NanoLuc-HA-β2AR-NNES-AsLOV2(V416L) | Expresses NanoLuc-β2AR-AsLOV2(V416L) in mammalian cells |
| 125226 | pAAV-mCherry-GS linker-Zdk1 | Expresses mCherry-GS linker-Zdk1 in mammalian cells |
| 125227 | pAAV-DRD1-NNES-eLOV-TEVcs-Flag-Gal4-V5 | Expresses SPARK DRD1 transmembrane component in mammalian cells |
| 125228 | pAAV-NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp | Expresses SPARK2 NanoLuc-βarrestin2-TEVp in mammalian cells |
| 125229 | pAAV-IgK sp-HA-CD4-10aa linker-CIBN-NNES-NanoLuc | Expresses transmembrane intracellular NanoLuc in mammalian cells |
| 125230 | pLX208-gLuc sp-NanoLuc-HA-oβ2AR-TS-eLOV-TEVcs-Flag-Gal4 | For generating SPARK NanoLuc-oβ2AR transmembrane component lentivirus |
| 125231 | pLX208-NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp | For generating SPARK2 NanoLuc-βarrestin2-TEVp lentivirus |
| 125232 | pFPGW-UAS-Citrine | For generating UAS-Citrine lentivirus |
| 125233 | pLX208-gLuc sp-NanoLuc-15aa linker-ICAM-Nrxn3b-HA | Expresses "sender" trans-cellular NanoLuc in mammalian cells |
| 125234 | pAAV-gLuc sp-GS linker-HiBit-Flag-oβ2AR-TS-NNES-eLOV-TEVcs-Flag-Gal4-V5 | Expresses SPARK oβ2AR transmembrane component in mammalian cells |
| 125235 | pAAV-NanoLuc-15aa linker-βarrestin2-no HA-GS linker-TEVp | Expresses SPARK2 NanoLuc-βarrestin2-TEVp (no HA) in mammalian cells |
| 125236 | pAAV-β2AR-NNES-NanoLuc-eLOV-TEVcs-Flag-Gal4-V5 | Expresses modified SPARK β2AR-NanoLuc transmembrane component in mammalian cells |