Capturing the Onset of PRC2-Mediated Repressive Domain Formation.
Oksuz O, Narendra V, Lee CH, Descostes N, LeRoy G, Raviram R, Blumenberg L, Karch K, Rocha PP, Garcia BA, Skok JA, Reinberg D
Mol Cell. 2018 Jun 21;70(6):1149-1162.e5. doi: 10.1016/j.molcel.2018.05.023. PubMed Article
Oksuz O, Narendra V, Lee CH, Descostes N, LeRoy G, Raviram R, Blumenberg L, Karch K, Rocha PP, Garcia BA, Skok JA, Reinberg D
Mol Cell. 2018 Jun 21;70(6):1149-1162.e5. doi: 10.1016/j.molcel.2018.05.023. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 131321 | EED-KO-gRNA-1 | gRNA to knockout EED. Use with EED-KO-gRNA-2 |
| 131322 | EED-KO-gRNA-2 | gRNA to knockout EED. Use with EED-KO-gRNA-1 |
| 131323 | Y365A or A365Y inducible rescue | gRNA to generate i-WT-r or i-MT-r systems |
| 131324 | A365Y-GFP Donor for iWTr EED | Donor to generate i-WT-r system |
| 131325 | Y365A-GFP Donor for iMTr EED | Donor to generate i-MT-r system |
| 131326 | EED-F97A | gRNA to generate F97A cage-mutant EED |
| 131327 | JARID2-KO-gRNA-2 | gRNA to knockout JARID2. Use with JARID2-KO-gRNA-1 |
| 131328 | JARID2-KO-gRNA-1 | gRNA to knockout JARID2. Use with JARID2-KO-gRNA-2 |
| 131329 | EZH2-Promoter-gRNA | gRNA to generate endogenously tagged -tetR-EZH2 |
| 131331 | SKOR1-gRNA-N-del | gRNA to delete a nucleation site near Skor1. Use with SKOR1-gRNA-C-del |
| 131332 | SKOR1-gRNA-C-del | gRNA to delete a nucleation site near Skor1. Use with SKOR1-gRNA-N-del |
| 131333 | LMX1B-gRNA-N-del | gRNA to delete a nucleation site near Lmx1b. Use with LMX1B-gRNA-C-del |
| 131334 | MTF2_KO_ex11_gRNA-4 | gRNA to knockout MTF2. This gRNA is also used in Haojie Li et al. Nature 2017. It targets exon 11 of MTF2. |
| 131335 | HoxD del control us | gRNA to delete control region near HoxD. Use with HoxD del control ds |
| 131336 | HoxD del control ds | gRNA to delete control region near HoxD. Use with HoxD del control us |
| 131337 | HoxD del us1 | gRNA to delete a nucleation site near HoxD. Use with HoxD del ds1 |
| 131338 | HoxD del ds1 | gRNA to delete a nucleation site near HoxD. Use with HoxD del us1 |
| 131339 | 5xtetO (tet3) Gene Desert | gRNA to insert 5xTet operator sequence into a gene desert on chromosome 5. |
| 131340 | 5xtetO HoxC 3' | gRNA to insert 5xTet operator sequence into downstream of HoxC cluster. |
| 131341 | HoxC-downstream-5xTetO Donor | Donor template to insert 5xTet operator sequence into downstream of HoxC cluster. |
| 131516 | LMX1B-gRNA-C-del | gRNA to delete a nucleation site near Lmx1b. Use with LMX1B-gRNA-N-del |