A modular cloning toolkit for genome editing in plants.
Hahn F, Korolev A, Sanjurjo Loures L, Nekrasov V
BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. PubMed Article
Hahn F, Korolev A, Sanjurjo Loures L, Nekrasov V
BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 125760 | pAK-EL-01 | Endlinker for tRNA-gRNA system when using only one guide |
| 125761 | pAK-EL-02 | Endlinker for tRNA-gRNA system when using only two guides |
| 125762 | pAK-EL-03 | Endlinker for tRNA-gRNA system when using only three guides |
| 125763 | pAK-EL-04 | Endlinker for tRNA-gRNA system when using only four guides |
| 125764 | pAK-EL-05 | Endlinker for tRNA-gRNA system when using only five guides |
| 125798 | pFH13 | Level0 SpCas9, wheat codon optimized |
| 125799 | pFH14 | Level0 SaCas9, Arabidopsis codon optimized |
| 125800 | pFH15 | Level0 StCas9, Arabidopsis codon optimized |
| 125801 | pFH16 | Level0 FnCas12a, rice codon optimized |
| 125803 | pFH17 | Level0 LbCas12a, rice codon optimized |
| 125808 | pFH22 | Level0 xCas9 3.7 |
| 125809 | pFH32 | Level0 SpCas9-NG, human codon optimized |
| 125810 | pFH45 | Level0 nSpCas9-ABE7.10 base changer, human codon optimized |
| 125811 | pFH46 | Level0 FnCas12a, human codon optimized |
| 125812 | pFH47 | Level0 LbCas12a, human codon optimized |
| 125813 | pFH48 | Level0 Csy4 |
| 125814 | pFH55 | Level0 nSpCas9-PmCDA, Arabidopsis codon optimized |
| 125815 | pFH76 | Level0 ScCas9, wheat codon optimized |
| 125816 | pFH77 | Level0 enCas9-PolI3M-TBD, wheat codon optimized |
| 125818 | pFH79 | Level0 A3A-PBE, wheat codon optimized |
| 125819 | pFH91 | Level0 nScCas9 (nickase), wheat codon optimized |
| 125820 | pFH92 | Level0 nScCas9-ABE7.10, wheat/human codon optimized |
| 125879 | pFH40 | Level0 Switchgrass PvUBI1 Pol II promoter |
| 125880 | pFH41 | Level0 Parsley PcUBI4-2 Pol II promoter |
| 125881 | pFH42 | Level0 Cestrum Yellow Leaf Curling Virus CmYLCV Pol II promoter |
| 125882 | pFH56 | Level0 Rice OsACT1 Pol II promoter |
| 125883 | pFH57 | Level0 Soybean GmUBI Pol II promoter |
| 125884 | pFH31 | Level0 Wheat TaU3 Pol III promoter |
| 125885 | pFH33 | Level0 Wheat TaU3 Pol III promoter with transcription Start-A |
| 126008 | pFH34 | Level0 Arabidopsis AtU6-26 Pol III promoter |
| 126009 | pFH35 | Level0 Arabidopsis AtU6-26 Pol III promoter with transcription Start-G |
| 126010 | pFH36 | Level0 Rice OsU6-2 Pol III promoter |
| 126011 | pFH37 | Level0 Rice OsU6-2 Pol III promoter with transcription Start-G |
| 126012 | pFH38 | Level0 Rice OsU3 Pol III promoter |
| 126013 | pFH39 | Level0 Rice OsU3 Pol III promoter with transcription Start-A |
| 126020 | pFH43 | Level0 Pea RbSC E9 terminator |
| 126021 | pFH44 | Level0 Pea 3A terminator |
| 128180 | pFH23 | Level1 ZmUBIp::SpCas9 (wheat codon optimized):NOSt |
| 128181 | pFH52 | Level1 2x35Sp::SpCas9 (Arabidopsis codon optimized):NOSt |
| 128182 | pFH53 | Level1 2x35Sp::SpCas9 (Arabidopsis codon optimized):pea3At |
| 128183 | pFH54 | Level1 AtUBI10p::SpCas9 (Arabidopsis codon optimized):pea3At |
| 128184 | pFH58 | Level1 ZmUBIp::SaCas9 (Arabidopsis codon optimized):NOSt |
| 128185 | pFH59 | Level1 ZmUBIp::StCas9 (Arabidopsis codon optimized):NOSt |
| 128186 | pFH60 | Level1 ZmUBIp::FnCas12a (rice codon optimized):NOSt |
| 128187 | pFH61 | Level1 ZmUBIp::LbCas12a (rice codon optimized):NOSt |
| 128188 | pFH68 | Level1 ZmUBIp::SpCas9-NG (human codon optimized):NOSt |
| 128189 | pFH69 | Level1 ZmUBIp::ABE7.10 base changer (human codon optimized):NOSt |
| 128190 | pFH70 | Level1 ZmUBIp::nSpCas9-PmCDA (Arabidopsis codon optimized):NOSt |
| 128191 | pFH80 | Level1 ZmUBIp::xCas9 3.7:NOSt |
| 128192 | pFH81 | Level1 ZmUBIp::ScCas9 (wheat codon optimized):NOSt |
| 128193 | pFH82 | Level1 ZmUBIp::enCas9-Poll3M-TBD (wheat codon optimized):NOSt |
| 128195 | pFH84 | Level1 ZmUBIp::A3A-PBE (wheat codon optimized):NOSt |
| 128196 | pFH93 | Level1 ZmUBIp::nScCas9-ABE7.10 (wheat/human codon optimized):NOSt |
| 128197 | pFH109 | Level1 ZmUBIp::nScCas9 (wheat codon optimized):NOSt |
| 128198 | pFH85 | classic sgRNA backbone for SpCas9, combine with TaU3 promoter module (pFH33) |
| 128199 | pFH86 | improved sgRNA backbone for SpCas9, combine with TaU3 promoter module (pFH33) |
| 128200 | pFH87 | sgRNA backbone for StCas9, combine with TaU3 promoter module (pFH33) |
| 128201 | pFH88 | sgRNA backbone for SaCas9, combine with TaU3 promoter module (pFH33) |
| 128202 | pFH89 | crRNA backbone for LbCas12a, combine with TaU3 promoter module (pFH33) |
| 128203 | pFH90 | crRNA backbone for FnCas12a/CmsI nucleases, combine with TaU3 promoter module (pFH33) |
| 128204 | pFH99 | classic sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35) |
| 128205 | pFH100 | improved sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35) |
| 128206 | pFH101 | sgRNA backbone for StCas9, combine with AtU6-26 promoter module (pFH35) |
| 128207 | pFH102 | sgRNA backbone for SaCas9, combine with AtU6-26 promoter module (pFH35) |
| 128208 | pFH103 | crRNA backbone for LbCas12a, combine with AtU6-26 promoter module (pFH35) |
| 128209 | pFH104 | crRNA backbone for FnCas12a/CmsI nucleases, combine with AtU6-26 promoter module (pFH35) |
| 128210 | pFH113 | improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU3 promoter module (pFH31) |
| 128211 | pFH49 | improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34) |
| 128212 | pFH50 | improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU6-2 promoter module (pFH36) |
| 128213 | pFH51 | improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU3 promoter module (pFH38) |
| 128214 | pFH71 | improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003) |
| 128215 | pAK002 | improved sgRNA backbone for tRNA-gRNA array, Position 2 |
| 128216 | pAK003 | improved sgRNA backbone for tRNA-gRNA array, Position 3 |
| 128217 | pAK004 | improved sgRNA backbone for tRNA-gRNA array, Position 4 |
| 128218 | pAK005 | improved sgRNA backbone for tRNA-gRNA array, Position 5 |
| 128219 | pAK006 | improved sgRNA backbone for tRNA-gRNA array, Position 6 |
| 128220 | pAK007 | classic sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU3 promoter module (pFH31) |
| 128221 | pFH72 | classic sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34) |
| 128222 | pFH73 | classic sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU6-2 promoter module (pFH36) |
| 128223 | pFH74 | classic sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU3 promoter module (pFH38) |
| 128224 | pFH75 | classic sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003) |
| 128225 | pAK008 | classic sgRNA backbone for tRNA-gRNA array, Position 2 |
| 128226 | pAK009 | classic sgRNA backbone for tRNA-gRNA array, Position 3 |
| 128227 | pAK010 | classic sgRNA backbone for tRNA-gRNA array, Position 4 |
| 128228 | pAK011 | classic sgRNA backbone for tRNA-gRNA array, Position 5 |
| 128229 | pAK012 | classic sgRNA backbone for tRNA-gRNA array, Position 6 |
| 128408 | pFH114 | Level1 ZmUBIp::BAR:NOSt |
| 131763 | pFH24 | Level0 SpCas9, wheat codon optimized (version 2) |
| 131764 | pFH25 | Level0 SpCas9, wheat codon optimized (version 3) |
| 131765 | pFH66 | Level1 ZmUBIp::SpCas9 (wheat codon optimized, version 2):NOSt |
| 131766 | pFH67 | Level1 ZmUBIp::SpCas9 (wheat codon optimized, version 3):NOSt |
| 136547 | pFH132 | Level1 ZmUBIp::FnCas12a (human codon optimized):NOSt |
| 136548 | pFH133 | Level1 ZmUBIp::LbCas12a (human codon optimized):NOSt |
| 136549 | pFH130 | crRNA backbone for FnCas12a/CmsI nucleases, combine with OsU6-2 promoter module (pFH37) |
| 136550 | pFH131 | crRNA backbone for FnCas12a/CmsI nucleases, combine with OsU3 promoter module (pFH39) |