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Lineage tracing and analog recording in mammalian cells by single-site DNA writing.
Loveless TB, Grotts JH, Schechter MW, Forouzmand E, Carlson CK, Agahi BS, Liang G, Ficht M, Liu B, Xie X, Liu CC
Nat Chem Biol. 2021 Jun;17(6):739-747. doi: 10.1038/s41589-021-00769-8. Epub 2021 Mar 22.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
126424pcDNA-Cas9-T2A-TdTExpresses Cas9-T2A-TdT in mammalian cells. (pTBL209)
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126425pcDNA-Cas9-T2A-STOP-TdTExpresses Cas9 but not TdT in mammalian cells; control for pcDNA-Cas9-T2A-TdT. (pTBL552)
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126426pTBL515 AA sgRNATo target a protospacer with AA at the cut site.
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126427pTBL402 AC sgRNATo target a protospacer with AC at the cut site.
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126428pTBL071 AG sgRNATo target a protospacer with AG at the cut site.
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126429pTBL509 AT sgRNATo target a protospacer with AT at the cut site.
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126430pTBL510 CA sgRNATo target a protospacer with CA at the cut site.
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126431pTBL511 CC sgRNATo target a protospacer with CC at the cut site.
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126432pTBL512 CG sgRNATo target a protospacer with CG at the cut site.
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126433pTBL516 CT sgRNATo target a protospacer with CT at the cut site.
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126434pTBL507 GA sgRNATo target a protospacer with GA at the cut site.
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126435pTBL401 GC sgRNATo target a protospacer with GC at the cut site.
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126436pTBL251 GG sgRNATo target a protospacer with GG at the cut site.
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126437pTBL063 GT sgRNATo target a protospacer with GT at the cut site.
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126438pTBL508 TA sgRNATo target a protospacer with TA at the cut site.
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126439pTBL250 TC sgRNATo target a protospacer with TC at the cut site.
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126440pTBL514 TG sgRNATo target a protospacer with TG at the cut site.
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126441pTBL513 TT sgRNATo target a protospacer with TT at the cut site.
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126442pTBL405 CHYRON1 integration constructTo integrate the CHYRON1 locus at HEK293site3.
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126445pTBL436 HEK293site3EQR sgRNATo cut the human HEK293 site3 locus for integration.
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126446pTBL649 CHYRON2 integration constructTo integrate the CHYRON2 locus at AAVS1 in human cells.
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126447pTBL437 AAVS1 EQR sgRNATo cut the human AAVS1 locus for integration.
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126448pTBL680 CHYRON3 integration constructTo integrate the CHYRON3 locus at AAVS1 in human cells.
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126449pTBL681 CHYRON4 integration constructTo integrate the CHYRON4 locus at AAVS1 in human cells.
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126450pcDNA-TdTExpresses human TdT in mammalian cells. (pTBL206)
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126451pTBL210Expresses Cas9-XTEN-TdT in mammalian cells.
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126454pJHG003Expresses Cas9-XTEN-dTdT in mammalian cells.
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126455pJHG004Expresses dTdT in mammalian cells.
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126457pTBL472Expresses Cas9-10xFlag-TdT in mammalian cells.
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126458pTBL469Expresses Cas9-15xFlag-TdT in mammalian cells.
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126477pTBL227 NheI-Cas9-KpnI-5xFlag-FseI-TdT-NotIExpresses Cas9-5xFlag-TdT in mammalian cells.
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126480pTBL235 NheI-Cas9-KpnI-GSAlinker-FseI-TdT-NotIExpresses Cas9-GSAlinker-TdT in mammalian cells.
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126950pTBL322 GCTo target a protospacer with GC at the cut site.
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126951pTBL581 GATo target a protospacer with GA at the cut site.
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126952pTBL601 AGTo target a protospacer with AG at the cut site.
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126953pTBL602 CGTo target a protospacer with CG at the cut site.
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132667pTBL716 4xHRE-YB-TATA-Cas9-ODD-T2A-TdTExpresses hypoxia-inducible Cas9 and TdT in mammalian cells.
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163643pTBL1269 pLenti-CHYRON17-pEF1a(short)-Luc2-P2A-tdTomato-T2A-TdTMakes a lentivirus that expresses hgRNA with 17nt spacer and Luc2, tdTomato, and human TdT.
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163644pTBL1401 pLenti-pEF1a(short)-mNeonGreen-P2A-Cas9Makes a lentivirus that expresses mNeonGreen and Cas9.
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Antibodies from Article