Lineage tracing and analog recording in mammalian cells by single-site DNA writing.
Loveless TB, Grotts JH, Schechter MW, Forouzmand E, Carlson CK, Agahi BS, Liang G, Ficht M, Liu B, Xie X, Liu CC
Nat Chem Biol. 2021 Jun;17(6):739-747. doi: 10.1038/s41589-021-00769-8. Epub 2021 Mar 22. PubMed Article
Loveless TB, Grotts JH, Schechter MW, Forouzmand E, Carlson CK, Agahi BS, Liang G, Ficht M, Liu B, Xie X, Liu CC
Nat Chem Biol. 2021 Jun;17(6):739-747. doi: 10.1038/s41589-021-00769-8. Epub 2021 Mar 22. PubMed Article
Plasmids from Article
ID | Plasmid | Purpose | |
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126424 | pcDNA-Cas9-T2A-TdT | Expresses Cas9-T2A-TdT in mammalian cells. (pTBL209) |
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126425 | pcDNA-Cas9-T2A-STOP-TdT | Expresses Cas9 but not TdT in mammalian cells; control for pcDNA-Cas9-T2A-TdT. (pTBL552) |
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126426 | pTBL515 AA sgRNA | To target a protospacer with AA at the cut site. |
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126427 | pTBL402 AC sgRNA | To target a protospacer with AC at the cut site. |
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126428 | pTBL071 AG sgRNA | To target a protospacer with AG at the cut site. |
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126429 | pTBL509 AT sgRNA | To target a protospacer with AT at the cut site. |
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126430 | pTBL510 CA sgRNA | To target a protospacer with CA at the cut site. |
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126431 | pTBL511 CC sgRNA | To target a protospacer with CC at the cut site. |
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126432 | pTBL512 CG sgRNA | To target a protospacer with CG at the cut site. |
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126433 | pTBL516 CT sgRNA | To target a protospacer with CT at the cut site. |
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126434 | pTBL507 GA sgRNA | To target a protospacer with GA at the cut site. |
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126435 | pTBL401 GC sgRNA | To target a protospacer with GC at the cut site. |
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126436 | pTBL251 GG sgRNA | To target a protospacer with GG at the cut site. |
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126437 | pTBL063 GT sgRNA | To target a protospacer with GT at the cut site. |
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126438 | pTBL508 TA sgRNA | To target a protospacer with TA at the cut site. |
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126439 | pTBL250 TC sgRNA | To target a protospacer with TC at the cut site. |
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126440 | pTBL514 TG sgRNA | To target a protospacer with TG at the cut site. |
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126441 | pTBL513 TT sgRNA | To target a protospacer with TT at the cut site. |
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126442 | pTBL405 CHYRON1 integration construct | To integrate the CHYRON1 locus at HEK293site3. |
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126445 | pTBL436 HEK293site3EQR sgRNA | To cut the human HEK293 site3 locus for integration. |
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126446 | pTBL649 CHYRON2 integration construct | To integrate the CHYRON2 locus at AAVS1 in human cells. |
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126447 | pTBL437 AAVS1 EQR sgRNA | To cut the human AAVS1 locus for integration. |
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126448 | pTBL680 CHYRON3 integration construct | To integrate the CHYRON3 locus at AAVS1 in human cells. |
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126449 | pTBL681 CHYRON4 integration construct | To integrate the CHYRON4 locus at AAVS1 in human cells. |
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126450 | pcDNA-TdT | Expresses human TdT in mammalian cells. (pTBL206) |
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126451 | pTBL210 | Expresses Cas9-XTEN-TdT in mammalian cells. |
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126454 | pJHG003 | Expresses Cas9-XTEN-dTdT in mammalian cells. |
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126455 | pJHG004 | Expresses dTdT in mammalian cells. |
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126457 | pTBL472 | Expresses Cas9-10xFlag-TdT in mammalian cells. |
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126458 | pTBL469 | Expresses Cas9-15xFlag-TdT in mammalian cells. |
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126477 | pTBL227 NheI-Cas9-KpnI-5xFlag-FseI-TdT-NotI | Expresses Cas9-5xFlag-TdT in mammalian cells. |
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126480 | pTBL235 NheI-Cas9-KpnI-GSAlinker-FseI-TdT-NotI | Expresses Cas9-GSAlinker-TdT in mammalian cells. |
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126950 | pTBL322 GC | To target a protospacer with GC at the cut site. |
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126951 | pTBL581 GA | To target a protospacer with GA at the cut site. |
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126952 | pTBL601 AG | To target a protospacer with AG at the cut site. |
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126953 | pTBL602 CG | To target a protospacer with CG at the cut site. |
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132667 | pTBL716 4xHRE-YB-TATA-Cas9-ODD-T2A-TdT | Expresses hypoxia-inducible Cas9 and TdT in mammalian cells. |
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163643 | pTBL1269 pLenti-CHYRON17-pEF1a(short)-Luc2-P2A-tdTomato-T2A-TdT | Makes a lentivirus that expresses hgRNA with 17nt spacer and Luc2, tdTomato, and human TdT. |
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163644 | pTBL1401 pLenti-pEF1a(short)-mNeonGreen-P2A-Cas9 | Makes a lentivirus that expresses mNeonGreen and Cas9. |
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