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Optimization of Insect Odorant Receptor Trafficking and Functional Expression Via Transient Transfection in HEK293 Cells.
Miazzi F, Hoyer C, Sachse S, Knaden M, Wicher D, Hansson BS, Lavista-Llanos S
Chem Senses. 2019 Oct 26;44(9):673-682. doi: 10.1093/chemse/bjz062.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
126472pcDNA3.1(-)-mRho.V5.mER.hOr47aHuman codon-optimized version of D. melanogaster Or47a (hOr47a) with N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved intracellular trafficking in mammalian cells
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126473pcDNA3.1(-)-V5.mER.hOr47aHuman codon-optimized version of D. melanogaster Or47a (hOr47a) with an N-terminal tag derived from human HCN1 (mER) for improved intracellular trafficking in mammalian cells
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126474pcDNA3.1(-)-V5.hOr47aHuman codon-optimized version of D. melanogaster Or47a (hOr47a) without N-terminal tags for improved intracellular trafficking in mammalian cells
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126475pCMV-BIHigh-copy number vector for the co-expression of two genes in mammalian cells
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126476pDmelORVector for the functional expression of insect ORs in mammalian cells, with a human codon-optimized version of D.melanogaster Orco (hOrco) with an N-terminal myc tag and a β-globin/IgG chimeric intron
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126478pDmelOR-mRho.V5.mER.hOr47aHuman codon-optimized D. melanogaster Orco (hOrco) and Or47a (hOr47a). Or47a has N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved trafficking in mammalian cells
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126479pDmelOR-mRho.V5.mER.hOr56aHuman codon-optimized D. melanogaster Orco (hOrco) and Or56a (hOr56a). Or56a has N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved trafficking in mammalian cells
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Antibodies from Article