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Spatial control of Draper receptor signaling initiates apoptotic cell engulfment.
Williamson AP, Vale RD
J Cell Biol. 2018 Nov 5;217(11):3977-3992. doi: 10.1083/jcb.201711175. Epub 2018 Aug 23.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
126653pMT Draper-GFPInducible expression of Draper-GFP in S2 cells
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126654pMT Draper-Ala11-GFPInducible expression of Draper-Ala11-GFP mutant in S2 cells
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126655pMT Draper-delta ITAM-GFPInducible expression of Draper-delta ITAM-GFP mutant in S2 cells
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126656pMT Draper-ITAM only-GFPInducible expression of Draper-ITAM only-GFP mutant in S2 cells
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126657pMT GFPInducible expression of GFP in S2 cells
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126658pMT Shark-TagBFPInducible expression of Shark-TagBFP in S2 cells
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126659pMT Crk-mCherryInducible expression of Crk-mCherry in S2 cells
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126660pMT p85-GFPInducible expression of p85-GFP in S2 cells
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126661pMT vCrk-GFPInducible expression of vCrk truncation in S2 cells
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126662pMT Stat92E-GFPInducible expression of Stat92E-GFP in S2 cells
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126663pMT Ptp69D-GFPInducible expression of Ptp69D-GFP in S2 cells
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126664pMT Lifeact-TagBFPInducible expression of Lifeact-TagBFP in S2 cells
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126665pMT FRB-Draper INT-GFPInducible expression of a synthetic FRB-Draper receptor in S2 cells
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126666pMT FRB-Draper INT-mCherryInducible expression of a synthetic FRB-Draper receptor in S2 cells
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126667pMT Draper-mCherryInducible expression of Draper-mCherry in S2 cells
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126668pMT Shark-mCherryInducible expression of Shark-mCherry in S2 cells
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126669pMT mCherry-CaaXInducible expression of mCherry-CaaX in S2 cells
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126670pET His10-Draper INTExpression of His10-Draper intracellular domain for purification from E. coli
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126671pET His10-Draper delta ITAMExpression of His10-Draper delta ITAM mutant for purification from E. coli
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126672pGEX Shark-tSH2Expression of Shark tandem SH2 domains SNAP tagged for use as FRET reporter
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126673pGEX Src42aExpression of GST-Src42a for purification from E. coli
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126674pET His10-Src42aExpression of His10-Src42a for purification from E. coli
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Antibodies from Article