A large-scale resource for tissue-specific CRISPR mutagenesis in Drosophila.
Port F, Strein C, Stricker M, Rauscher B, Heigwer F, Zhou J, Beyersdorffer C, Frei J, Hess A, Kern K, Lange L, Langner N, Malamud R, Pavlovic B, Radecke K, Schmitt L, Voos L, Valentini E, Boutros M
Elife. 2020 Feb 13;9. pii: 53865. doi: 10.7554/eLife.53865. PubMed Article
Port F, Strein C, Stricker M, Rauscher B, Heigwer F, Zhou J, Beyersdorffer C, Frei J, Hess A, Kern K, Lange L, Langner N, Malamud R, Pavlovic B, Radecke K, Schmitt L, Voos L, Valentini E, Boutros M
Elife. 2020 Feb 13;9. pii: 53865. doi: 10.7554/eLife.53865. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 127382 | UAS-u(XS)Cas9 | Expresses Cas9 at very high level |
| 127383 | UAS-u(S)Cas9 | Expresses Cas9 at high level |
| 127384 | UAS-u(M)Cas9 | Expresses Cas9 at medium level |
| 127385 | UAS-u(L)Cas9 | Expresses Cas9 at low level |
| 127386 | UAS-u(XL)Cas9 | Expresses Cas9 at very low level |
| 127387 | UAS-u(XXL)Cas9 | Expresses Cas9 at very low level |
| 127388 | UAS-FRT-GFP-FRT-u(M)Cas9 | Cas9 inducible by removal of a GFP flip-out cassette |