Imaging-based pooled CRISPR screening reveals regulators of lncRNA localization.
Wang C, Lu T, Emanuel G, Babcock HP, Zhuang X
Proc Natl Acad Sci U S A. 2019 May 28;116(22):10842-10851. doi: 10.1073/pnas.1903808116. Epub 2019 May 13. PubMed Article
Wang C, Lu T, Emanuel G, Babcock HP, Zhuang X
Proc Natl Acad Sci U S A. 2019 May 28;116(22):10842-10851. doi: 10.1073/pnas.1903808116. Epub 2019 May 13. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 127396 | pFUGW Cas9-BFP | Cas9 expression |
| 127397 | library backbone 3'LTR gRNA and barcode | Digestion by BstXI and BamHI to release the backbone |