Target preference of Type III-A CRISPR-Cas complexes at the transcription bubble.
Liu TY, Liu JJ, Aditham AJ, Nogales E, Doudna JA
Nat Commun. 2019 Jul 5;10(1):3001. doi: 10.1038/s41467-019-10780-2. PubMed Article
Liu TY, Liu JJ, Aditham AJ, Nogales E, Doudna JA
Nat Commun. 2019 Jul 5;10(1):3001. doi: 10.1038/s41467-019-10780-2. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 127764 | pACYC-TtCas6-4xcrRNA4.5 | For expression of T. thermophilus Csm complex in E. coli (crRNA) |
| 128572 | pCDF-5xT7-TtCsm | For expression of T. thermophilus Csm in E. coli (Csm1-5) |