Mark Groudine Lab:
Plasmids for generating AID/Degron Cell Lines in Human and Mouse
Unpublished
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 127897 | Mouse 3' Hp1a AID GFP PuroR | HDR (donor) Plasmid for Inserting AID-GFP-2A-Puro into the 3' end of the Mouse Hp1a Gene |
| 127898 | pX330 Mouse 3' Hp1a gRNA | WT Cas9 Vector targeting the 3' end of the mouse Hp1a gene |
| 127899 | Mouse 5' Npm1 AID GFP PuroR | HDR (donor) Plasmid for Inserting PuroR-2A-GFP-AID into the 5' end of the Mouse Npm1 Gene |
| 127900 | pX330 Mouse 5' Npm1 gRNA | WT Cas9 Vector targeting the 5' end of the mouse Npm1 gene |
| 127901 | Mouse 3' Ubf AID GFP PuroR | HDR (donor) Plasmid for Inserting AID-GFP-2A-Puro into the 3' end of the Mouse Ubf Gene |
| 127902 | pX330 Mouse 3' Ubf gRNA | WT Cas9 Vector targeting the 3' end of the mouse Ubf gene |
| 127903 | Mouse 5' Srcap AID GFP Puro | HDR (donor) Plasmid for Inserting PuroR-2A-GFP-AID into the 5' end of the Mouse Srcap Gene |
| 127904 | pX335 Mouse 5' Srcap gRNA A | Cas9 D10A Nickase Vector targeting the 5' end of the mouse Srcap gene |
| 127905 | pX335 Mouse 5' Srcap gRNA B | Cas9 D10A Nickase Vector targeting the 5' end of the mouse Srcap gene |
| 127906 | Human 3' HP1a AID GFP PuroR | HDR (donor) Plasmid for Inserting AID-GFP-2A-Puro into the 3' end of the Human HP1a Gene |
| 127907 | pX330 Human 3' HP1a gRNA | WT Cas9 Vector targeting the 3' end of the human HP1a gene |
| 127908 | pSpCas9(BB)-2A-Blast_HP1beta_gRNA | WT Cas9 Vector with Blasticidin Selection Marker targeting the 5' end of the human HP1beta gene |
| 127909 | SB100X in pCAG globin pA | Sleeping Beauty Transposase expression vector driven by the CAG promoter |
| 127910 | pEf1a OsTIR ires NEO pA T2BH | Transposon vector containing an OsTIR IRES Neo cassette driven by the Ef1a promoter |