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Proximity-CLIP provides a snapshot of protein-occupied RNA elements in subcellular compartments.
Benhalevy D, Anastasakis DG, Hafner M
Nat Methods. 2018 Dec;15(12):1074-1082. doi: 10.1038/s41592-018-0220-y. Epub 2018 Nov 26.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
107596pEXP(V5-APEX2-NES)Expresses V5-APEX2-NES in mammalian cells
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107597pEXP(V5_H2B_APEX2)Expresses V5_H2B_APEX2 in mammallian cells
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168207pDEST(frt)(ATG_V5-APEX2_noSTOP)Destination vector for GATEWAY cloning, N-terminal V5-APEX2, Tet operator in the CMV promoter (Tet ON), FRT sequence for T-Rex stable expression, Hygromycin selection
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Antibodies from Article