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CRISPR-Cas12b enables efficient plant genome engineering.
Ming M, Ren Q, Pan C, He Y, Zhang Y, Liu S, Zhong Z, Wang J, Malzahn AA, Wu J, Zheng X, Zhang Y, Qi Y
Nat Plants. 2020 Mar;6(3):202-208. doi: 10.1038/s41477-020-0614-6. Epub 2020 Mar 9. PubMed Article

Plasmids from Article

ID Plasmid Purpose
129670pYPQ290Gateway entry clone for CRISPR-Cas12b systems
129671pYPQ291Gateway entry clone for CRISPR-Cas12b systems
129672pYPQ292Gateway entry clone for CRISPR-Cas12b systems
129673pYPQ290-D570AGateway entry clone for CRISPR-Cas12b systems
129674pYPQ290-D977AGateway entry clone for CRISPR-Cas12b systems
129675pYPQ290-E848AGateway entry clone for CRISPR-Cas12b systems
129676pYPQ291-D573AGateway entry clone for CRISPR-Cas12b systems
129677pYPQ291-D951AGateway entry clone for CRISPR-Cas12b systems
129678pYPQ291-E827AGateway entry clone for CRISPR-Cas12b systems
129679pYPQ292-D570AGateway entry clone for CRISPR-Cas12b systems
129680pYPQ292-D977AGateway entry clone for CRISPR-Cas12b systems
129681pYPQ292-E848AGateway entry clone for CRISPR-Cas12b systems
129682pYPQ290-D570A-SRDXGateway entry clone for CRISPR-Cas12b systems
129683pYPQ291-D573A-SRDXGateway entry clone for CRISPR-Cas12b systems
129684pYPQ292-D570A-SRDXGateway entry clone for CRISPR-Cas12b systems
129685pYPQ141-ZmUbi-RZ-Aac(Empty backbone) Gateway entry clone with sgRNA under ZmUbi promoter
129686pYPQ141-ZmUbi-RZ-Bth(Empty backbone) Gateway entry clone with sgRNA under ZmUbi promoter
136372pYPQ141-ZmUbi-RZ-Aa1.2.3Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 1.2 with MS2 at the third stem loop is used
136373pYPQ141-ZmUbi-RZ-Aa1.2Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 1.2 is used
136374pYPQ141-ZmUbi-RZ-Aa3.8.3Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 3.8 with MS2 at the third stem loop is used
136375pYPQ141-ZmUbi-RZ-Aa3.8.4Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 3.8 with MS2 at the third stem loop is used
136376pYPQ141-ZmUbi-RZ-Aa3.8Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 3.8 is used
136377pYPQ141-ZmUbi-RZ-Aac.3Gateway entry clone for AacCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold with MS2 at the third stem loop is used
136378pYPQ141-ZmUbi-RZ-BhGateway entry clone for BhCas12b sgRNA expression under ZmUbi promoter with ribozyme processing
136379pYPQ239A (dFnCas12a)-TVCRISPRa Gateway entry clone for catalytically dead FnCas12a fused with TV activation system
136380pYPQ292 (AaCas12b)-D570A-TVCRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system
136381pYPQ292 (AaCas12b)-D570A-TV-MS2-TVCRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-TV fusion protein
136382pYPQ292 (AaCas12b)-D570A-TV-MS2-VPRCRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-VPR fusion protein
136383pYPQ293 (BhCas12b_v4)Gateway entry clone for BhCas12b_v4

Antibodies from Article