A cell death assay in barley and wheat protoplasts for identification and validation of matching pathogen AVR effector and plant NLR immune receptors.
Saur IML, Bauer S, Lu X, Schulze-Lefert P
Plant Methods. 2019 Oct 24;15:118. doi: 10.1186/s13007-019-0502-0. eCollection 2019. PubMed Article
Saur IML, Bauer S, Lu X, Schulze-Lefert P
Plant Methods. 2019 Oct 24;15:118. doi: 10.1186/s13007-019-0502-0. eCollection 2019. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 131860 | pZmUBQ:LUC | Transient expression of firefly luciferase from the Zea mays ubiquitin promotor |
| 132355 | pZmUBQ:Mla1 | Expression of the barley Mla1 NLR from the Zea mays ubiquitin promotor |
| 132356 | pZmUBQ:AVRa1 | Expression of the Blumeria graminis f. sp. hordei effector AVRa1 ∆SP from the Zea mays ubiquitin promotor |
| 132357 | pZmUBQ:AVRa1-V1 | Expression of the Blumeria graminis f. sp. hordei effector AVRa1-V1 ∆SP from the Zea mays ubiquitin promotor |
| 132358 | pZmUBQ:GW | Expression of CDS from the Zea mays ubiquitin promotor |