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Strong gene activation in plants with genome-wide specificity using a new orthogonal CRISPR/Cas9-based programmable transcriptional activator.
Selma S, Bernabe-Orts JM, Vazquez-Vilar M, Diego-Martin B, Ajenjo M, Garcia-Carpintero V, Granell A, Orzaez D
Plant Biotechnol J. 2019 Sep;17(9):1703-1705. doi: 10.1111/pbi.13138. Epub 2019 May 23. PubMed Article

Plasmids from Article

ID Plasmid Purpose
160558pVD1 M1_3-pTRNA-scf 2.1 (GB2073)tRNA and scaffold 2.1scRNA aptamer Ms2 for the assembly of GBoligomers for the first position (positon [M1_3]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.
160559pVD1 M2_3-pTRNA-scf 2.1 (GB2074)tRNA and scaffold 2.1scRNA aptamer Ms2 for the assembly of GBoligomers for the first position (positon [M2_3]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter
160560pVD1 M3_3-pTRNA-scf 2.1 (GB2075)tRNA and scaffold 2.1scRNA aptamer Ms2 for the assembly of GBoligomers for the first position (positon [M3_3]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter
160568pVD1 M1_1-pTRNA-scf 2.1 (GB2603)tRNA and scaffold 2.1 scRNA aptamer Ms2 for the assembly of GBoligomers for a single position of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.
160570pUPD2 sgRNA:scaffold tetraloop MS2 aptamer SAM (GB1436)A version of scaffold sgRNA with the sequence of MS2 aptamer inside the tetraloop of the scaffold
160571pUPD2 sgRNAscaffold 2.1 scRNA (GB1437)Version of SgRNA scaffold with the sequence 2.1 of Ms2 aptamer in the 3' end.
160593pUPD2 sgRNA sCRNA 2.0 (GB2461)Version of the native Cas9-sgRNA with one native WT aptamer sequence and F6 aptamer sequence recognized for Ms2 coat protein, in 3'.
160609pDGB3 alpha2 P35S:MS2-VP64:Tnos (GB1395)TU for the constitutive expresion of the viral coat protein MS2 fused on C-terminal with the VP64
160621pDGB3 alpha1 U6-26:gRNA 4 (Pnos):2.1 aptamer (GB1724)Target for the Nopaline Synthetase Promoter with the MS2 recognition 2.1 loop in position3' in the scaffold
160622pDGB3 alpha2 P35S:MS2:EDLL:Tnos (GB1738)TU for the constitutive expression of the MS2 coat protein fused on Ct to the activation domain EDLL
160623pDGB3 alpha2 P35S:dCas9:VPR:Tnos (GB1826)TU for the constitutive expression of dCas9 fused to VPR (VP64-p65-Rta) activation domains
160624pDGB3 alpha2 P35S:MS2:VPR:Tnos (GB1830)TU for the constitutive expression of the MS2 coat protein fused on Ct to the activation domain VPR (VP64+p65+Rta)
160625pDGB3 alpha1 U6-26-1gRNA-DFR 2.1 (GB1838)GB-cassette for the expression of a guide RNA targeting the DFR promoter with 2.1 Ms2 aptamer in the 3' of the scaffold
160626pDGB3 alpha2 P35S:dCas9:TV:Tnos (GB2047)TU for the constitutive expression of dCas9 fused to TV (TALx6 - VP128) activation domains
160627pDGB3 alpha2 P35S:MS2:TV:Tnos (GB2048)TU for the constitutive expression of Ms2 fused to TV (TALx6 - VP128) activation domains
160645pDGB3 omega1 P35S:MS2:VPR:Tnos - P35S:dCas9:EDLL:Tnos (GB2085)Module for the expression of Ms2 protein fused to VPR and dCas9 fused to EDLL

Antibodies from Article