Synaptotagmin 1 clamps synaptic vesicle fusion in mammalian neurons independent of complexin.
Courtney NA, Bao H, Briguglio JS, Chapman ER
Nat Commun. 2019 Sep 9;10(1):4076. doi: 10.1038/s41467-019-12015-w. PubMed Article
Courtney NA, Bao H, Briguglio JS, Chapman ER
Nat Commun. 2019 Sep 9;10(1):4076. doi: 10.1038/s41467-019-12015-w. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 133815 | pFSW GFP IRES GFP | Encodes EGFP that can be used as a control for viral expression |
| 133816 | pFSW Syt1 WT IRES GFP | Encodes full-length wild type synaptotagmin 1 for viral expression |
| 133817 | pFSW Syt1-C2B IRES GFP | Encodes the C2B domain of synaptotagmin 1 for viral expression |
| 133818 | pFSW Syt1-C2A IRES GFP | Encodes the C2A domain of synaptotagmin 1 for viral expression |
| 133819 | pFSW Syt1-C2B R398,399Q IRES GFP | Encodes the C2B domain of synaptotagmin 1 with mutations R398,399Q for viral expression |
| 133820 | pFSW Syt1-C2B K326,327E IRES GFP | Encodes the C2B domain of synaptotagmin 1 with mutations K326,327E for viral expression |
| 133821 | pFSW Syt1-C2B V304A,I367A IRES GFP | Encodes the C2B domain of synaptotagmin 1 with membrane-penetrating residue mutations V304A,I367A for viral expression |
| 133822 | pFSW Syt1-C2B D363,365N IRES GFP | Encodes the C2B domain of synaptotagmin 1 with calcium-coordinating ligand mutations D363,365N for viral expression |
| 133823 | pFSW Syt1-C2A K189-192A IRES GFP | Encodes the C2A domain of synaptotagmin 1 with poly-lysine patch mutations K189-192A for viral expression |
| 133824 | pFSW Syt1-C2A D230,232N IRES GFP | Encodes the C2A domain of synaptotagmin 1 with calcium-coordinating ligand mutations D230,232N for viral expression |
| 133825 | pFSW Syt1-C2A M173,F234A IRES GFP | Encodes the C2A domain of synaptotagmin 1 with membrane-penetrating residue mutations M173,F234A for viral expression |