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High-Performance Intensiometric Direct- and Inverse-Response Genetically Encoded Biosensors for Citrate.
Zhao Y, Shen Y, Wen Y, Campbell RE
ACS Cent Sci. 2020 Aug 26;6(8):1441-1450. doi: 10.1021/acscentsci.0c00518. Epub 2020 Jul 9.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
134300pBAD-Citron1Bacterial expression of direct-response citrate sensor
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134301pBAD-Citroff1Bacterial expression of inverse-response citrate sensor with high binding affinity
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134302pBAD-Citroff1-S245Bacterial expression of an inverse-response citrate sensor variant with medium binding affinity
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134303CMV-Citron1Mammalian expression of direct-response citrate sensor
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134304CMV-Citroff1Mammalian expression of inverse-response citrate sensor
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134305CMV-mito-Citron1mitochondria-targeted direct-response citrate sensor
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134306CMV-mito-Citroff1-S245mitochondria-targeted inverse-response citrate sensor variant with medium binding affinity
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134307pBAD-Citroff1-R251Bacterial expression of an inverse-response citrate sensor variant with medium binding affinity
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158545CMV-CitronRHMammalian expression of nonbinding variant of direct-response citrate sensor
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158546CMV-CitroffRHMammalian expression of nonbinding variant of inverse-response citrate sensor
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158547CMV-mito-CitronRHMitochondria-targeted nonbinding variant of direct-response citrate sensor
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158548CMV-mito-CitroffRHMitochondria-targeted nonbinding variant of inverse-response citrate sensor
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Antibodies from Article