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Broad-spectrum anti-CRISPR proteins facilitate horizontal gene transfer.
Mahendra C, Christie KA, Osuna BA, Pinilla-Redondo R, Kleinstiver BP, Bondy-Denomy J
Nat Microbiol. 2020 Apr;5(4):620-629. doi: 10.1038/s41564-020-0692-2. Epub 2020 Mar 26.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
133788pCAG-hSt3Cas9-NLS(SV40)-3xFLAG (KAC426)CAG promoter expression plasmid for human codon optimized St3Cas9 nuclease with C-terminal NLS (SV40)
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133789pCAG-hCjeCas9-NLS(SV40)-3xFLAG (KAC579)CAG promoter expression plasmid for human codon optimized CjeCas9 nuclease with C-terminal NLS (SV40)
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133790pCAG-hNmeCas9-NLS(SV40)-3xFLAG (KAC409)CAG promoter expression plasmid for human codon optimized NmeCas9 nuclease with C-terminal NLS (SV40)
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133791pUC19-U6-BsmBI_cassette-St1Cas9-sgRNA (KAC14)U6 promoter sgRNA entry vector used for all St1Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V1 sgRNA architecture from Carter et al. biorxiv 2018
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133792pUC19-U6-BsmBI_cassette-St3Cas9-sgRNA (KAC27)U6 promoter sgRNA entry vector used for all St3Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Muller et al. Molecular Therapy 2015
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133793pUC19-U6-BsmBI_cassette-CjeCas9-sgRNA (KAC482)U6 promoter sgRNA entry vector used for all CjeCas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V2-TGAA linker sgRNA architecture from Kim et al. Nature Communications 2017
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133794pUC19-U6-BsmBI_cassette-Nme/Nme2Cas9-sgRNA (KAC32)U6 promoter sgRNA entry vector used for all NmeCas9 or Nme2Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Amrani et al. Genome Biology 2018
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133801pCMV-T7-AcrIIA4-NLS(SV40) (KAC200)CMV and T7 promoter expression plasmid for human codon optimized AcrIIA4 with C-terminal NLS (SV40)
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133802pCMV-T7-AcrIIA5-NLS(SV40) (KAC203)CMV and T7 promoter expression plasmid for human codon optimized AcrIIA5 with C-terminal NLS (SV40)
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134331pCAG-hNme2Cas9-NLS(SV40)-3xFLAG (KAC582)CAG promoter expression plasmid for human codon optimized Nme2Cas9 nuclease with C-terminal NLS (SV40) and 3x FLAG tag
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134332pCMV-T7-AcrIIA16_Lmo-NLS(SV40) (KAC508)CMV and T7 promoter expression plasmid for human codon optimized AcrIIA16_Lmo with C-terminal NLS (SV40)
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134333pCMV-T7-AcrIIA17_Efa-NLS(SV40) (KAC91)CMV and T7 promoter expression plasmid for human codon optimized AcrIIA17_Efa with C-terminal NLS (SV40)
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134334pCMV-T7-AcrIIA17_Sga-NLS(SV40) (KAC92)CMV and T7 promoter expression plasmid for human codon optimized AcrIIA17_Sga with C-terminal NLS (SV40)
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134335pCMV-T7-AcrIIA18_Sma-NLS(SV40) (KAC95)CMV and T7 promoter expression plasmid for human codon optimized AcrIIA18_Sma with C-terminal NLS (SV40)
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134336pCMV-T7-AcrIIA19_Ssim-NLS(SV40) (KAC97)CMV and T7 promoter expression plasmid for human codon optimized AcrIIA19_Ssim with C-terminal NLS (SV40)
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134337pCMV-T7-AcrIIA16_Efa-NLS(SV40) (KAC459)CMV and T7 promoter expression plasmid for human codon optimized AcrIIA16_Efa with C-terminal NLS (SV40)
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134338pCMV-T7-AcrIIA18_Sga-NLS(SV40) (KAC214)CMV and T7 promoter expression plasmid for human codon optimized AcrIIA18_Sga with C-terminal NLS (SV40)
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134339pCMV-T7-AcrIIA19_Spse-NLS(SV40) (KAC215)CMV and T7 promoter expression plasmid for human codon optimized AcrIIA19_Spse with C-terminal NLS (SV40)
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134340pCMV-T7-AcrIIC1-NLS(SV40) (KAC208)CMV and T7 promoter expression plasmid for human codon optimized AcrIIC1 with C-terminal NLS (SV40)
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134341pCMV-T7-AcrIIC3-NLS(SV40) (KAC209)CMV and T7 promoter expression plasmid for human codon optimized AcrIIC3 with C-terminal NLS (SV40)
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Antibodies from Article