CRISPR-READI: Efficient Generation of Knockin Mice by CRISPR RNP Electroporation and AAV Donor Infection.
Chen S, Sun S, Moonen D, Lee C, Lee AY, Schaffer DV, He L
Cell Rep. 2019 Jun 25;27(13):3780-3789.e4. doi: 10.1016/j.celrep.2019.05.103. PubMed Article
Chen S, Sun S, Moonen D, Lee C, Lee AY, Schaffer DV, He L
Cell Rep. 2019 Jun 25;27(13):3780-3789.e4. doi: 10.1016/j.celrep.2019.05.103. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 135616 | pscAAV-Tyr | Donor vector for genomic targeting of an EcoRI restriction site to the mouse Tyrosinase locus |
| 135617 | pscAAV-Sox2-mStr | Donor vector for genomic targeting of a P2A-mStrawberry cassette to the 3' end of the mouse Sox2 locus |
| 135618 | pAAV-Sox2-CreERT2 | Donor vector for genomic targeting of a P2A-CreERT2 cassette to the 3' end of the mouse Sox2 locus |
| 135619 | pAAV-R26-FLEX-mStr | Donor vector for genomic targeting of a CAG-FLEX-mStrawberry cassette to the mouse Rosa26 locus |