Recruitment of DNA Repair MRN Complex by Intrinsically Disordered Protein Domain Fused to Cas9 Improves Efficiency of CRISPR-Mediated Genome Editing.
Reuven N, Adler J, Broennimann K, Myers N, Shaul Y
Biomolecules. 2019 Oct 8;9(10). pii: biom9100584. doi: 10.3390/biom9100584. PubMed Article
Reuven N, Adler J, Broennimann K, Myers N, Shaul Y
Biomolecules. 2019 Oct 8;9(10). pii: biom9100584. doi: 10.3390/biom9100584. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 135010 | pU6-(BsaI)_CBh-Cas9 | Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 |
| 135011 | pU6-(BsaI)_CBh-UN-Cas9 | Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to N-terminus of Cas9 |
| 135012 | pU6-(BsaI)_CBh-Cas9-T2A-mCherry | Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 linked to mCherry via a T2A peptide |
| 135013 | pU6-(BsaI)_CBh-UN-Cas9-T2A-mCherry | Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to N-terminus of Cas9, linked to mCherry via a T2A peptide |
| 135014 | pU6-(BsaI)_CBh-UC-Cas9-T2A-mCherry | Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to C-terminus of Cas9, linked to mCherry via a T2A peptide |