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Genetic modification of primary human B cells to model high-grade lymphoma.
Caeser R, Di Re M, Krupka JA, Gao J, Lara-Chica M, Dias JML, Cooke SL, Fenner R, Usheva Z, Runge HFP, Beer PA, Eldaly H, Pak HK, Park CS, Vassiliou GS, Huntly BJP, Mupo A, Bashford-Rogers RJM, Hodson DJ
Nat Commun. 2019 Oct 4;10(1):4543. doi: 10.1038/s41467-019-12494-x.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
135305MSCV-BCL6-t2A-BCL2Overexpression of multiple human genes (BCL2 and BCL6)
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135306MSCV-MYC-t2A-BCL2Overexpression of multiple human genes (BCL2 and MYC)
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163611MSCV-BCL2Overexpression of human BCL2
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163612phCMV-GALV-MTRRetro and Lentiviral transduction of primary human germinal center B cells
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164662MSCV-Cas9-2A-BFPRetroviral introduction of Cas9 into mammalian cell line coupled to expression of blue fluorescent protein
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Antibodies from Article