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krCRISPR: an easy and efficient strategy for generating conditional knockout of essential genes in cells.
Wang B, Wang Z, Wang D, Zhang B, Ong SG, Li M, Yu W, Wang Y
J Biol Eng. 2019 Apr 24;13:35. doi: 10.1186/s13036-019-0150-y. eCollection 2019.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
135970KO plasmidExpresses SpCas9, and cloning backbone for sgRNA
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135971rescue plasmidexpresses copGFP and puromycin resistance genes
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Antibodies from Article