Optimization of AsCas12a for combinatorial genetic screens in human cells.
DeWeirdt PC, Sanson KR, Sangree AK, Hegde M, Hanna RE, Feeley MN, Griffith AL, Teng T, Borys SM, Strand C, Joung JK, Kleinstiver BP, Pan X, Huang A, Doench JG
Nat Biotechnol. 2020 Jul 13. pii: 10.1038/s41587-020-0600-6. doi: 10.1038/s41587-020-0600-6. PubMed Article
DeWeirdt PC, Sanson KR, Sangree AK, Hegde M, Hanna RE, Feeley MN, Griffith AL, Teng T, Borys SM, Strand C, Joung JK, Kleinstiver BP, Pan X, Huang A, Doench JG
Nat Biotechnol. 2020 Jul 13. pii: 10.1038/s41587-020-0600-6. doi: 10.1038/s41587-020-0600-6. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 136474 | pRDA_052 | Lentiviral expression of AsCas12a gRNA |
| 136475 | pRDA_112 | Lentiviral expression of AsCas12a |
| 136476 | pRDA_174 | Lentiviral expression of EnAsCas12a |
| 136477 | pRosetta_v2 | Lentiviral GFP Reporter assay for determining appropriate dose of puromycin, blasticidin, and hygromycin |
| 172650 | Humagne Set C | Set C contains best-ranked guides 1 and 4. |
| 172651 | Humagne Set D | Set D contains best-ranked guides 2 and 3. |