Titrating gene expression using libraries of systematically attenuated CRISPR guide RNAs.
Jost M, Santos DA, Saunders RA, Horlbeck MA, Hawkins JS, Scaria SM, Norman TM, Hussmann JA, Liem CR, Gross CA, Weissman JS
Nat Biotechnol. 2020 Jan 13. pii: 10.1038/s41587-019-0387-5. doi: 10.1038/s41587-019-0387-5. PubMed Article
Jost M, Santos DA, Saunders RA, Horlbeck MA, Hawkins JS, Scaria SM, Norman TM, Hussmann JA, Liem CR, Gross CA, Weissman JS
Nat Biotechnol. 2020 Jan 13. pii: 10.1038/s41587-019-0387-5. doi: 10.1038/s41587-019-0387-5. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 136478 | Large-scale mismatched sgRNA library for gene titration (human essential genes, CRISPRi) | Expresses gRNAs for gene titration of ~2400 human genes using CRISPRi |
| 136479 | Compact mismatched sgRNA library for gene titration (human essential genes, CRISPRi) | Expresses gRNAs for gene titration of ~2400 human genes using CRISPRi |