Continuous evolution of SpCas9 variants compatible with non-G PAMs.
Miller SM, Wang T, Randolph PB, Arbab M, Shen MW, Huang TP, Matuszek Z, Newby GA, Rees HA, Liu DR
Nat Biotechnol. 2020 Apr;38(4):471-481. doi: 10.1038/s41587-020-0412-8. Epub 2020 Feb 10. PubMed Article
Miller SM, Wang T, Randolph PB, Arbab M, Shen MW, Huang TP, Matuszek Z, Newby GA, Rees HA, Liu DR
Nat Biotechnol. 2020 Apr;38(4):471-481. doi: 10.1038/s41587-020-0412-8. Epub 2020 Feb 10. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 136918 | pCMV-BE4max-NRRH | Expresses BE4max-NRRH in mammalian cells |
| 136919 | pCMV-BE4max-NRTH | Expresses BE4max-NRTH in mammalian cells |
| 136920 | pCMV-BE4max-NRCH | Expresses BE4max-NRCH in mammalian cells |
| 136922 | pCMV-ABEmax-NRTH | Expresses ABEmax-NRTH in mammalian cells |
| 136923 | pCMV-ABEmax-NRCH | Expresses ABEmax-NRCH in mammalian cells |
| 136924 | pCMV-Cas9-NRRH | Expresses Cas9-NRRH in mammalian cells |
| 136925 | pCMV-Cas9-NRTH | Expresses Cas9-NRTH in mammalian cells |
| 136926 | pCMV-Cas9-NRCH | Expresses Cas9-NRCH in mammalian cells |
| 136927 | pTW211b5 | Directed evolution of SpCas9 |
| 136928 | pTW199b2 | Directed evolution of SpCas9 |
| 136929 | pTW170b2 | Directed evolution of SpCas9 |
| 136930 | pTW194b63 | Directed evolution of SpCas10 |
| 136931 | pTW195b63 | Directed evolution of SpCas11 |