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IL-4 controls activated neutrophil FcgammaR2b expression and migration into inflamed joints.
Panda SK, Wigerblad G, Jiang L, Jimenez-Andrade Y, Iyer VS, Shen Y, Boddul SV, Guerreiro-Cacais AO, Raposo B, Kasza Z, Wermeling F
Proc Natl Acad Sci U S A. 2020 Jan 24. pii: 1914186117. doi: 10.1073/pnas.1914186117.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
137729LentiGuide Puro-P2A-EGFPFor simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).
137730LentiGuide-Puro-P2A-EGFP_mRFPstufExpresses customizable S. Pyogenes sgRNA from U6 promoter and PuroR-P2A-EGFP from EF-1a promoter. Stuffer contains mRFP from a bacterial promoter, enabling simple visual quality control step of sgRNA

Antibodies from Article