A fast genetically encoded fluorescent sensor for faithful in vivo acetylcholine detection in mice, fish, worms and flies
Borden PM, Zhang P, Shivange AV, Marvin JS, Cichon J, Dan C, Podgorski K, Figueiredo A, Novak O, Tanimoto M, Shigetomi E, Lobas MA, Kim H, Zhu PK, Zhang Y, Zheng WS, Fan C, Wang G, Xiang B, Gan L, Zhang G, Guo K, Lin L, Cai Y, Yee AG, Aggarwal A, Ford CP, Rees DC, Dietrich D, Khakh BS, Dittman JS, Gan W, Koyama M, Jayaraman V, Cheer JF, Lester HA, Zhu JJ, Looger LL
bioRxiv 2020 Article
Borden PM, Zhang P, Shivange AV, Marvin JS, Cichon J, Dan C, Podgorski K, Figueiredo A, Novak O, Tanimoto M, Shigetomi E, Lobas MA, Kim H, Zhu PK, Zhang Y, Zheng WS, Fan C, Wang G, Xiang B, Gan L, Zhang G, Guo K, Lin L, Cai Y, Yee AG, Aggarwal A, Ford CP, Rees DC, Dietrich D, Khakh BS, Dittman JS, Gan W, Koyama M, Jayaraman V, Cheer JF, Lester HA, Zhu JJ, Looger LL
bioRxiv 2020 Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 137950 | pAAV.hSynap.iAChSnFR | Expresses iAChSnFR under hSynapsin promoter |
| 137951 | pAAV.hSynap.iAChSnFR-NULL | Expresses iAChSnFR (non-binding) under hSynapsin promoter |
| 137953 | pAAV.hSynap.iAChSnFR-Venus-NULL | Expresses iAChSnFR (non-binding yellow version) under hSynapsin promoter |
| 137955 | pAAV.CAG.iAChSnFR | Expresses iAChSnFR under CAG promoter |
| 137956 | pAAV.CAG.iAChSnFR-NULL | Expresses iAChSnFR (non-binding variant) under CAG promoter |
| 137959 | pAAV.CAG-FLEX.iAChSnFR-Venus | Expresses FLEXed iAChSnFR (yellow version) under CAG promoter |
| 137960 | pAAV.CAG-FLEX.iAChSnFR-Venus-NULL | Expresses FLEXed iAChSnFR (non-binding yellow version) under CAG promoter |
| 137962 | pAAV.ZAC.GFAP.iAChSnFR | Expresses iAChSnFR under GFAP promoter |
| 140643 | pSinREP5-iAchSnFR | Acetylcholine sensor for sindbis virus |
| 140644 | pLenti-hSynapsin-iAchSnFR | Acetylcholine sensor for lenti virus |