Genomic Characterization of Endothelial Enhancers Reveals a Multifunctional Role for NR2F2 in Regulation of Arteriovenous Gene Expression.
Sissaoui S, Yu J, Yan A, Li R, Zhu LJ, Kucukural A, Yukselen O, Lawson ND
Circ Res. 2020 Feb 17. doi: 10.1161/CIRCRESAHA.119.316075. PubMed Article
Sissaoui S, Yu J, Yan A, Li R, Zhu LJ, Kucukural A, Yukselen O, Lawson ND
Circ Res. 2020 Feb 17. doi: 10.1161/CIRCRESAHA.119.316075. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 138354 | pME-minP:luc2 | Gateway compatible middle entry clone contains Firefly Luciferase2 insert driven by minimal promoter |
| 138355 | pME-luc2 | Gateway compatible middle entry clone contains Firefly Luciferase2 without minimal promoter |
| 138356 | p5E-CMVSP6 | Gateway compatible 5' entry clone contains Cytomegalovirus and SP6 dual promoter(CMVSSP6) |
| 138358 | p3E-SV40-puromycin | Gateway compatible 3' entry clone contains puromycin driven by simian virus 40 (SV40) |
| 138359 | pME-NR2F2 | Gateway compatible middle entry clone contains human full length NFR2F2 gene with stop codon |
| 138361 | pLenti-CMVSP6-NR2F2-SV40-PURO | Lentiviral plasmid contains human NR2F2 driven by CMVSP6 and puromycin resistance for selection |
| 138362 | p5E-mcs2 | Gateway compatible 5' entry clone contains mutli-cloning sites sequence2. |
| 138363 | pME-nEGFP | Gateway compatible middle entry clone contains nuclear EGFP sequences. |
| 138364 | pLenti-CMVSP6-nEGFP-SV40-PURO | Lentiviral plasmid for nuclear EGFP expression driven by CMVSP6 and puromycin resistance under SV40 promoter for selection |
| 138365 | p5E-161K enhancer | Gateway compatible 5' entry clone contains human modified 161k enhancer sequence. |
| 138367 | pLenti-161K enhancer-ETS-CORE-MUT-minP:fLuc-TK:rLuc | Lentiviral plasmid contains 161k modified human enhancer with with ETS binding sites mutated driving firefly Luciferase 2 and Renilla Luciferase driven by TK promoter |
| 138368 | pLenti-161K enhancer-NR2F2-MUT-minP:fLuc-TK:rLuc | Lentiviral plasmid contains 161k modified human enhancer with with NR2F2 binding sites mutated driving firefly Luciferase 2 and Renilla Luciferase driven by TK promoter |
| 138369 | pLenti-basP:fLuc-TK:rLuc | Lentiviral plasmid contains basal promoter driving firefly Luciferase 2 and Renilla Luciferase driven by TK promoter |
| 138371 | p5E-161K enhancer-NR2F2-MUT | Gateway compatible 5' entry clone contains human modified 161k enhancer sequence. with NR2F2 binding sites mutated |
| 138372 | p5E-161K enhancer-ETS-CORE-MUT | Gateway compatible 5' entry clone contains human modified 161k enhancer sequence. with ETS binding sites mutated |