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An improved strategy for CRISPR/Cas9 gene knockout and subsequent wildtype and mutant gene rescue.
Jin J, Xu Y, Huo L, Ma L, Scott AW, Pizzi MP, Li Y, Wang Y, Yao X, Song S, Ajani JA
PLoS One. 2020 Feb 13;15(2):e0228910. doi: 10.1371/journal.pone.0228910. eCollection 2020.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
140206pLentiCrispr-V2-mOrange (V2mO)Lenti Crispr/Cas9 with mOrange fluorescent marker
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Antibodies from Article