Genomic Profiling by ALaP-Seq Reveals Transcriptional Regulation by PML Bodies through DNMT3A Exclusion.
Kurihara M, Kato K, Sanbo C, Shigenobu S, Ohkawa Y, Fuchigami T, Miyanari Y
Mol Cell. 2020 May 7;78(3):493-505.e8. doi: 10.1016/j.molcel.2020.04.004. Epub 2020 Apr 29. PubMed Article
Kurihara M, Kato K, Sanbo C, Shigenobu S, Ohkawa Y, Fuchigami T, Miyanari Y
Mol Cell. 2020 May 7;78(3):493-505.e8. doi: 10.1016/j.molcel.2020.04.004. Epub 2020 Apr 29. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 140273 | pBlue-loxPuro-APEX-PML_LASA | a targeting plasmid for APEX-PML |
| 140279 | pGbH500-Nanog-AXP | a targeting plasmid for Nanog-APEX |
| 140280 | pX300A-G+Nanog | CRISPR/Cas9 plasmid encoding Cas9 and sgRNA against gBait and Nanog locus |
| 140281 | pEF-APEX2-NLS | Expression of APEX2-NLS |
| 140283 | pGb_PMLlxP_EGFP | a targeting plasmid for EGFP-PML |
| 140284 | pCAG-Cre-iBSD | Expression of Cre-IRES-BSD |
| 140285 | pYMCR2_Gb_PMLTSS | CRISPR/Cas9 plasmids encoding sgRNA targeting TSS of PML for knock-in |
| 140286 | pCFL-mPML-2CAMut-iB | Expression of mouse PML Ca mutant |
| 140287 | pCFL-mPML-3KRmut-iB | Expression of mouse PML KR mutant |