Multistable and dynamic CRISPRi-based synthetic circuits.
Santos-Moreno J, Tasiudi E, Stelling J, Schaerli Y
Nat Commun. 2020 Jun 2;11(1):2746. doi: 10.1038/s41467-020-16574-1. PubMed Article
Santos-Moreno J, Tasiudi E, Stelling J, Schaerli Y
Nat Commun. 2020 Jun 2;11(1):2746. doi: 10.1038/s41467-020-16574-1. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 140664 | pJ1996_v2 | dCas9 & Csy4 |
| 140665 | pJ2018 | dCas9, LuxR & Csy4 |
| 140666 | pJ2077.2 | Controller plasmid for TS circuit and cL and cR controls |
| 140667 | pJ2076.2_TS | Toggle switch (TS) |
| 140668 | pJ2076.2_cL | cL control for toggle switch experiment |
| 140669 | pJ2076.2_cR | cR control for toggle switch experiment |
| 140670 | pJ2042.2 | 3-color stripe |
| 140671 | pJ2042.2_Bs | 1-color stripe (“BLUE”) |
| 140672 | pJ2048.2_Gs | 1-color stripe (“GREEN”) |
| 140673 | pJ2048_2xNOT | Double-inverter |
| 140674 | pJ2072.2_CRISPRlator | CRISPRlator |
| 140675 | pJ2072.2_c1 | CRISPRlator control (“open ring”) |
| 140676 | pJ2044 | NOT gate with sg-1 |
| 140677 | pJ2043 | NOT gate with sg-2 |
| 140678 | pJ2039 | NOT gate with sg-3 |
| 140679 | pJ2040 | NOT gate with sg-4 |
| 140680 | pJ2042.2_Brocc | Broccoli Stripe |
| 140681 | pJ2043_N2only | Control (“C”) lacking sg-2 |
| 140682 | pJ2039_N2only | Control (“C”) lacking sg-3 |
| 140683 | pJ2040_N2only | Control (“C”) lacking sg-4 |
| 140684 | pJ2044_t4 | NOT gate with sg-1t4 |
| 140685 | pJ2040_t4 | NOT gate with sg-4t4 |
| 140686 | pJ2042.2_GFPonly | 1-color stripe |
| 140687 | pJ2048.2 | 3-color stripe (different sgRNAs) |
| 140688 | pJ2042.2_invRep | 3-color stripe (inverted reporters) |
| 140689 | pJ2044_N2only | Control (“C”) lacking sg-1 |