In vivo cell biological screening identifies an endocytic capture mechanism for T-tubule formation.
Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim YW, Parton RG
Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w. PubMed Article
Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim YW, Parton RG
Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 87120 | p5E-4xnrUAS | Multisite gateway entry clone containing 4 non-repetitive UAS (upstream activation sequence) elements |
| 109487 | actc1b-mKate2-CaaX(tH) | Muscle specific zebrafish expression of mKate2 fused to the CaaX domain from Hras |
| 109488 | actc1b-mKate2-CaaX(tK) | Muscle specific zebrafish expression of mKate2 fused to the CaaX domain from Kras |
| 109490 | actc1b-mKate2-GPI-pA | Muscle specific zebrafish expression of mKate2 fused to the 5' and 3' sequences from zebrafish folate receptor 1 |
| 109491 | actc1b-mKate2-MTM1 | Muscle specific zebrafish expression of mKate2 fused to myotubularin |
| 109492 | actc1b-mKate2-DysF | Muscle specific zebrafish expression of mKate2 fused to dysferlin |
| 109493 | actc1b-Lifeact-mKate2 | Muscle specific zebrafish expression of mKate2 fused to the actin binding lifeact sequence |
| 109494 | actc1b- mKate2-KDEL-pA | Muscle specific zebrafish expression of mKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequence |
| 109496 | actc1b-TCAP-mKate2 | Muscle specific zebrafish expression of mKate2 fused to telethonin |
| 109497 | actc1b-mKate2-jph1a | Muscle specific zebrafish expression of mKate2 fused to junctophilin 1a |
| 109498 | actc1b-mKate2-SERCA1 | Muscle specific zebrafish expression of mKate2 fused to sarcoplasmic/endoplasmic calcium ATPase 1 |
| 109499 | actc1b-mKate2-LactC2 | Muscle specific zebrafish expression of mKate2 fused to the C-terminal domain from cow (Bos taurus) milk fat globue EGF-factor 8 protein (MFGE8). Binds phosphatidylserine (PS) |
| 109500 | actc1b-2PH-PLCd-mKate2 | Muscle specific zebrafish expression of mKate2 fused to 2x the PH domain from Human phospholipase C delta 1 (PLCD1, codon optimised). Binds PtdIns(4,5)P2. |
| 109501 | actc1b-BTK-mKate2 | Muscle specific zebrafish expression of mKate2 fused to the PH domain from Human Bruton tyrosine kinase (BTK). Binds PtdIns(3,4,5)P3. |
| 109502 | actc1b-mKate2-ING2 | Muscle specific zebrafish expression of mKate2 fused to 3xPHD domain from Mouse inhibitor of growth family member 2 (ING2), codon optimised. Binds PtdIns5P |
| 109503 | actc1b-mKate2-FAPP1 | Muscle specific zebrafish expression of mKate2 fused to the PH domain from Human pleckstrin homology domain containing A3 (PLEKHA3). Binds PtdIns4P |
| 109504 | actc1b-mKate2-ATG18 | Muscle specific zebrafish expression of mKate2 fused to yeast ATG18 (PLEKHA3). Binds PtdIns(3,5P)2 |
| 109505 | actc1b-mKate2-2xFYVEhgs | Muscle specific zebrafish expression of mKate2 fused to mouse 2x PH domain from HGF regulated tyrosine kinase substrate (Hgs). Binds PtdIns3P |
| 109506 | actc1b-GalT-mKate2 | Muscle specific zebrafish expression of mKate2 fused to the N-terminal sequence from zebrafish 1,4 galactosyltransferase 1 (GalT). Marks the Golgi complex. |
| 109507 | actc1b-lamp1-mKate2 | Muscle specific zebrafish expression of mKate2 fused to lamp1 |
| 109508 | actc1b-mKate2-EEA1 | Muscle specific zebrafish expression of mKate2 fused to early endosome associated 1 |
| 109509 | actc1b-mKate2-Bin1b | Muscle specific zebrafish expression of mKate2 fused to Bin1b |
| 109512 | actc1b-mKate2-EHD1a | Muscle specific zebrafish expression of mKate2 fused to EHD1a |
| 109513 | actc1b-mKate2-EHD1a-dEH | Muscle specific zebrafish expression of mKate2 fused to EHD1a-delta-EH |
| 109514 | actc1b-mKate2-EHD1b | Muscle specific zebrafish expression of mKate2 fused to EHD1b |
| 109515 | actc1b-mKate2-EHD1b-dEH | Muscle specific zebrafish expression of mKate2 fused to EHD1b-delta-EH |
| 109516 | actc1b-mKate2-EHD2a | Muscle specific zebrafish expression of mKate2 fused to EHD2a |
| 109517 | actc1b-mKate2-EHD2b | Muscle specific zebrafish expression of mKate2 fused to EHD2b |
| 109518 | actc1b-Cav3-mKate2 | Muscle specific zebrafish expression of mKate2 fused to Caveolin3 |
| 109519 | actc1b-mKate2-Dnm2a | Muscle specific zebrafish expression of mKate2 fused to Dynamin2a |
| 109520 | actc1b-mKate2-Dnm2a-DN | Muscle specific zebrafish expression of mKate2 fused to dominant negative Dynamin2a |
| 109521 | actc1b-mKate2-Dnm2b | Muscle specific zebrafish expression of mKate2 fused to Dynamin2b |
| 109522 | actc1b-mKate2-Dnm2b-DN | Muscle specific zebrafish expression of mKate2 fused to dominant negative Dynamin2b |
| 109523 | actc1b-CD44a-mKate2 | Muscle specific zebrafish expression of mKate2 fused to CD44a |
| 109524 | actc1b-CD44b-mKate2 | Muscle specific zebrafish expression of mKate2 fused to CD44b |
| 109525 | actc1b-mKate2-RhoAa | Muscle specific zebrafish expression of mKate2 fused to RhoAa |
| 109526 | actc1b-mKate2-RhoAa-DN | Muscle specific zebrafish expression of mKate2 fused to dominant negative RhoAa |
| 109527 | actc1b-mKate2-RhoAb | Muscle specific zebrafish expression of mKate2 fused to RhoAb |
| 109529 | actc1b-mKate2-Rac1 | Muscle specific zebrafish expression of mKate2 fused to Rac1 |
| 109530 | actc1b-mKate2-Rac1-DN | Muscle specific zebrafish expression of mKate2 fused to dominant negative Rac1 |
| 109531 | actc1b-mKate2-CDC42 | Muscle specific zebrafish expression of mKate2 fused to CDC42 |
| 109532 | actc1b-mKate2-CDC42-DN | Muscle specific zebrafish expression of mKate2 fused to dominant negative CDC42 |
| 109533 | actc1b-mKate2-Pacsin2 | Muscle specific zebrafish expression of mKate2 fused to Pacsin2 |
| 109535 | actc1b-mKate2-ARF1 | Muscle specific zebrafish expression of mKate2 fused to ARF1 |
| 109536 | actc1b-mKate2-ARF1-DN | Muscle specific zebrafish expression of mKate2 fused to dominant negative ARF1 |
| 109537 | actc1b-mKate2-ARF6 | Muscle specific zebrafish expression of mKate2 fused to ARF6 |
| 109538 | actc1b-mKate2-ARF6-DN | Muscle specific zebrafish expression of mKate2 fused to dominant negative ARF6 |
| 109539 | p3E-CaaX(tH) | Multisite gateway vector for 3' tagging with the CaaX domain from Hras |
| 109540 | p3E-CaaX(tK) | Multisite gateway vector for 3' tagging with the CaaX domain from Kras |
| 109541 | pME-myristo | Multisite gateway vector for 5' tagging with the 5' myristoylation sequence from ARF5 |
| 109542 | pME-mKate2-GPI-pA | Multisite gateway vector for expression of mKate2 fused to the 5' and 3' sequences from zebrafish folate receptor 1 |
| 109544 | p3E-DysF | Multisite gateway vector for 3' tagging with Dysferlin |
| 109545 | pME-Lifeact | Multisite gateway vector for 5' tagging with the actin binding motif Lifeact |
| 109546 | pME-mKate2-KDEL-pA | Multisite gateway vector for expression of mKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequence (marks the ER, or ER/SR in muscle) |
| 109547 | pME-nCas9n-P2A-mKate2-pA | Multisite gateway vector for expression of nCas9n with bicistronic expression of mKate2 |
| 109549 | pME-TCAP | Multisite gateway vector for 5' tagging with telethonin |
| 109550 | pME-actn2 | Multisite gateway vector for 5' tagging with alpha actinin 2 |
| 109551 | p3E-jph1a | Multisite gateway vector for 3' tagging with junctophilin 1a |
| 109552 | p3E-SERCA1 | Multisite gateway vector for 3' tagging with sarcoplasmic/endoplasmic calcium ATPase 1 |
| 109553 | p3E-Lact-C2 | Multisite gateway vector for 3' tagging with the C-terminal domain from cow (Bos taurus) milk fat globue EGF-factor 8 protein (MFGE8). Binds phosphatidylserine (PS) |
| 109554 | p3E-ING2 | Multisite gateway vector for 3' tagging with 3xPHD domain from Mouse inhibitor of growth family member 2 (ING2), codon optimised. Binds PtdIns5P |
| 109555 | p3E-FAPP1 | Multisite gateway vector for 3' tagging with the PH domain from Human pleckstrin homology domain containing A3 (PLEKHA3). Binds PtdIns4P |
| 109556 | p3E-ATG18 | Multisite gateway vector for 3' tagging with yeast ATG18 (PLEKHA3). Binds PtdIns(3,5P)2 |
| 109558 | pME-GalT | Multisite gateway vector for 5' tagging with the N-terminal sequence from zebrafish 1,4 galactosyltransferase 1 (GalT). Marks the Golgi complex. |
| 109559 | pME-lamp1 | Multisite gateway vector for 5' tagging with lamp1 |
| 109560 | p3E-EEA1 | Multisite gateway vector for 5' tagging with early endosome associated 1 |
| 109561 | p3E-Bin1b | Multisite gateway vector for 5' tagging with Bin1b |
| 109564 | p3E-EHD1a | Multisite gateway vector for 3' tagging with EHD1a |
| 109565 | p3E-EHD1a-dEH | Multisite gateway vector for 3' tagging with EHD1a-delta-EH |
| 109566 | p3E-EHD1b | Multisite gateway vector for 3' tagging with EHD1b |
| 109567 | p3E-EHD1b-dEH | Multisite gateway vector for 3' tagging with EHD1b-delta-EH |
| 109568 | p3E-EHD2a | Multisite gateway vector for 3' tagging with EHD2a |
| 109569 | p3E-EHD2b | Multisite gateway vector for 3' tagging with EHD2b |
| 109570 | pME-Cav3 | Multisite gateway vector for 5' tagging with Caveaolin3 |
| 109571 | p3E-Dnm2a | Multisite gateway vector for 3' tagging with Dynamin2a |
| 109572 | p3E-Dnm2a-DN | Multisite gateway vector for 3' tagging with dominant negative Dynamin2a |
| 109573 | p3E-Dnm2b | Multisite gateway vector for 3' tagging with Dynamin2b |
| 109574 | p3E-Dnm2b-DN | Multisite gateway vector for 3' tagging with dominant negative Dynamin2b |
| 109575 | pME-CD44a | Multisite gateway vector for 5' tagging with CD44a |
| 109577 | p3E-RhoAa | Multisite gateway vector for 3' tagging with RhoAa |
| 109579 | p3E-RhoAb | Multisite gateway vector for 3' tagging with RhoAb |
| 109580 | p3E-RhoAb-DN | Multisite gateway vector for 3' tagging with dominant negative RhoAb |
| 109581 | p3E-Rac1 | Multisite gateway vector for 3' tagging with Rac1 |
| 109582 | p3E-Rac1-DN | Multisite gateway vector for 3' tagging with dominant negative Rac1 |
| 109583 | p3E-CDC42 | Multisite gateway vector for 3' tagging with CDC42 |
| 109584 | p3E-CDC42-DN | Multisite gateway vector for 3' tagging with dominant negative CDC42 |
| 109585 | p3E-Clta | Multisite gateway vector for 3' tagging with Clathrin light chain a |
| 109586 | p3E-Cltb | Multisite gateway vector for 3' tagging with Clathrin light chain b |
| 109587 | p3E-Pacsin2 | Multisite gateway vector for 3' tagging with Pacsin2 |
| 109589 | p3E-ARF1 | Multisite gateway vector for 3' tagging with ARF1 |
| 109590 | p3E-ARF1-DN | Multisite gateway vector for 3' tagging with dominant negative ARF1 |
| 109591 | p3E-ARF6 | Multisite gateway vector for 3' tagging with ARF6 |
| 109592 | p3E-ARF6-DN | Multisite gateway vector for 3' tagging with dominant negative ARF6 |
| 109593 | actc1b-mKate2-rab10 | Muscle specific zebrafish expression of mKate2 fused to rab10 |
| 109594 | actc1b-mKate2-rab11a | Muscle specific zebrafish expression of mKate2 fused to rab11a |
| 109595 | actc1b-mKate2-rab11al | Muscle specific zebrafish expression of mKate2 fused to rab11al |
| 109596 | actc1b-mKate2-rab11ba | Muscle specific zebrafish expression of mKate2 fused to rab11ba |
| 109597 | actc1b-mKate2-rab11bb | Muscle specific zebrafish expression of mKate2 fused to rab11bb |
| 109598 | actc1b-mKate2-rab12 | Muscle specific zebrafish expression of mKate2 fused to rab12 |
| 109599 | actc1b-mKate2-rab13 | Muscle specific zebrafish expression of mKate2 fused to rab13 |
| 109600 | actc1b-mKate2-rab14 | Muscle specific zebrafish expression of mKate2 fused to rab14 |
| 109601 | actc1b-mKate2-rab14l | Muscle specific zebrafish expression of mKate2 fused to rab14l |
| 109602 | actc1b-mKate2-rab15 | Muscle specific zebrafish expression of mKate2 fused to rab15 |
| 109603 | actc1b-mKate2-rab18a | Muscle specific zebrafish expression of mKate2 fused to rab18a |
| 109604 | actc1b-mKate2-rab18b | Muscle specific zebrafish expression of mKate2 fused to rab18b |
| 109605 | actc1b-mKate2-rab1aa | Muscle specific zebrafish expression of mKate2 fused to rab1aa |
| 109606 | actc1b-mKate2-rab1ab | Muscle specific zebrafish expression of mKate2 fused to rab1ab |
| 109607 | actc1b-mKate2-rab1ba | Muscle specific zebrafish expression of mKate2 fused to rab1ba |
| 109608 | actc1b-mKate2-rab1bb | Muscle specific zebrafish expression of mKate2 fused to rab1bb |
| 109609 | actc1b-mKate2-rab20 | Muscle specific zebrafish expression of mKate2 fused to rab20 |
| 109610 | actc1b-mKate2-rab21 | Muscle specific zebrafish expression of mKate2 fused to rab21 |
| 109611 | actc1b-mKate2-rab22a | Muscle specific zebrafish expression of mKate2 fused to rab22a |
| 109612 | actc1b-mKate2-rab23 | Muscle specific zebrafish expression of mKate2 fused to rab23 |
| 109613 | actc1b-mKate2-rab24 | Muscle specific zebrafish expression of mKate2 fused to rab24 |
| 109614 | actc1b-mKate2-rab25a | Muscle specific zebrafish expression of mKate2 fused to rab25a |
| 109615 | actc1b-mKate2-rab25b | Muscle specific zebrafish expression of mKate2 fused to rab25b |
| 109616 | actc1b-mKate2-rab26 | Muscle specific zebrafish expression of mKate2 fused to rab26 |
| 109617 | actc1b-mKate2-rab27a | Muscle specific zebrafish expression of mKate2 fused to rab27a |
| 109618 | actc1b-mKate2-rab28 | Muscle specific zebrafish expression of mKate2 fused to rab28 |
| 109619 | actc1b-mKate2-rab2a | Muscle specific zebrafish expression of mKate2 fused to rab2a |
| 109620 | actc1b-mKate2-rab30 | Muscle specific zebrafish expression of mKate2 fused to rab30 |
| 109621 | actc1b-mKate2-rab32a | Muscle specific zebrafish expression of mKate2 fused to rab32a |
| 109622 | actc1b-mKate2-rab32b | Muscle specific zebrafish expression of mKate2 fused to rab32b |
| 109623 | actc1b-mKate2-rab33a | Muscle specific zebrafish expression of mKate2 fused to rab33a |
| 109624 | actc1b-mKate2-rab33ba | Muscle specific zebrafish expression of mKate2 fused to rab33ba |
| 109625 | actc1b-mKate2-rab34a | Muscle specific zebrafish expression of mKate2 fused to rab34a |
| 109626 | actc1b-mKate2-rab34b | Muscle specific zebrafish expression of mKate2 fused to rab34b |
| 109628 | actc1b-mKate2-rab35b | Muscle specific zebrafish expression of mKate2 fused to rab35b |
| 109629 | actc1b-mKate2-rab36 | Muscle specific zebrafish expression of mKate2 fused to rab36 |
| 109631 | actc1b-mKate2-rab38a | Muscle specific zebrafish expression of mKate2 fused to rab38a |
| 109632 | actc1b-mKate2-rab39a | Muscle specific zebrafish expression of mKate2 fused to rab39a |
| 109633 | actc1b-mKate2-rab39ba | Muscle specific zebrafish expression of mKate2 fused to rab39ba |
| 109634 | actc1b-mKate2-rab39bb | Muscle specific zebrafish expression of mKate2 fused to rab39bb |
| 109635 | actc1b-mKate2-rab3aa | Muscle specific zebrafish expression of mKate2 fused to rab3aa |
| 109636 | actc1b-mKate2-rab3ab | Muscle specific zebrafish expression of mKate2 fused to rab3ab |
| 109637 | actc1b-mKate2-rab3c | Muscle specific zebrafish expression of mKate2 fused to rab3c |
| 109638 | actc1b-mKate2-rab3da | Muscle specific zebrafish expression of mKate2 fused to rab3da |
| 109639 | actc1b-mKate2-rab3db | Muscle specific zebrafish expression of mKate2 fused to rab3db |
| 109640 | actc1b-mKate2-rab40b | Muscle specific zebrafish expression of mKate2 fused to rab40b |
| 109641 | actc1b-mKate2-rab40c | Muscle specific zebrafish expression of mKate2 fused to rab40c |
| 109642 | actc1b-mKate2-rab42a | Muscle specific zebrafish expression of mKate2 fused to rab42a |
| 109643 | actc1b-mKate2-rab42b | Muscle specific zebrafish expression of mKate2 fused to rab42b |
| 109645 | actc1b-mKate2-rab44 | Muscle specific zebrafish expression of mKate2 fused to rab44 |
| 109646 | actc1b-mKate2-rab4a | Muscle specific zebrafish expression of mKate2 fused to rab4a |
| 109647 | actc1b-mKate2-rab4b | Muscle specific zebrafish expression of mKate2 fused to rab4b |
| 109648 | actc1b-mKate2-rab5aa | Muscle specific zebrafish expression of mKate2 fused to rab5aa |
| 109649 | actc1b-mKate2-rab5ab | Muscle specific zebrafish expression of mKate2 fused to rab5ab |
| 109650 | actc1b-mKate2-rab5b | Muscle specific zebrafish expression of mKate2 fused to rab5b |
| 109651 | actc1b-mKate2-rab5c | Muscle specific zebrafish expression of mKate2 fused to rab5c |
| 109652 | actc1b-mKate2-rab6a | Muscle specific zebrafish expression of mKate2 fused to rab6a |
| 109653 | actc1b-mKate2-rab6ba | Muscle specific zebrafish expression of mKate2 fused to rab6ba |
| 109654 | actc1b-mKate2-rab6bb | Muscle specific zebrafish expression of mKate2 fused to rab6bb |
| 109655 | actc1b-mKate2-rab7a | Muscle specific zebrafish expression of mKate2 fused to rab7a |
| 109656 | actc1b-mKate2-rab7b | Muscle specific zebrafish expression of mKate2 fused to rab7b |
| 109657 | actc1b-mKate2-rab8a | Muscle specific zebrafish expression of mKate2 fused to rab8a |
| 109658 | actc1b-mKate2-rab8b | Muscle specific zebrafish expression of mKate2 fused to rab8b |
| 109659 | actc1b-mKate2-rab9a | Muscle specific zebrafish expression of mKate2 fused to rab9a |
| 109660 | actc1b-mKate2-rab9b | Muscle specific zebrafish expression of mKate2 fused to rab9b |
| 109661 | p3E-rab10 | Multisite gateway vector for 3' tagging with rab10 |
| 109662 | p3E-rab11a | Multisite gateway vector for 3' tagging with rab11a |
| 109663 | p3E-rab11al | Multisite gateway vector for 3' tagging with rab11al |
| 109664 | p3E-rab11ba | Multisite gateway vector for 3' tagging with rab11ba |
| 109665 | p3E-rab11bb | Multisite gateway vector for 3' tagging with rab11bb |
| 109666 | p3E-rab12 | Multisite gateway vector for 3' tagging with rab12 |
| 109667 | p3E-rab13 | Multisite gateway vector for 3' tagging with rab13 |
| 109668 | p3E-rab14 | Multisite gateway vector for 3' tagging with rab14 |
| 109669 | p3E-rab14l | Multisite gateway vector for 3' tagging with rab14l |
| 109670 | p3E-rab15 | Multisite gateway vector for 3' tagging with rab15 |
| 109671 | p3E-rab18a | Multisite gateway vector for 3' tagging with rab18a |
| 109672 | p3E-rab18b | Multisite gateway vector for 3' tagging with rab18b |
| 109673 | p3E-rab1aa | Multisite gateway vector for 3' tagging with rab1aa |
| 109674 | p3E-rab1ab | Multisite gateway vector for 3' tagging with rab1ab |
| 109675 | p3E-rab1ba | Multisite gateway vector for 3' tagging with rab1ba |
| 109676 | p3E-rab1bb | Multisite gateway vector for 3' tagging with rab1bb |
| 109677 | p3E-rab20 | Multisite gateway vector for 3' tagging with rab20 |
| 109678 | p3E-rab21 | Multisite gateway vector for 3' tagging with rab21 |
| 109679 | p3E-rab22a | Multisite gateway vector for 3' tagging with rab22a |
| 109680 | p3E-rab23 | Multisite gateway vector for 3' tagging with rab23 |
| 109681 | p3E-rab24 | Multisite gateway vector for 3' tagging with rab24 |
| 109682 | p3E-rab25a | Multisite gateway vector for 3' tagging with rab25a |
| 109683 | p3E-rab25b | Multisite gateway vector for 3' tagging with rab25b |
| 109685 | p3E-rab27a | Multisite gateway vector for 3' tagging with rab27a |
| 109686 | p3E-rab28 | Multisite gateway vector for 3' tagging with rab28 |
| 109687 | p3E-rab2a | Multisite gateway vector for 3' tagging with rab2a |
| 109688 | p3E-rab30 | Multisite gateway vector for 3' tagging with rab30 |
| 109689 | p3E-rab32a | Multisite gateway vector for 3' tagging with rab32a |
| 109690 | p3E-rab32b | Multisite gateway vector for 3' tagging with rab32b |
| 109691 | p3E-rab33a | Multisite gateway vector for 3' tagging with rab33a |
| 109692 | p3E-rab33ba | Multisite gateway vector for 3' tagging with rab33ba |
| 109693 | p3E-rab34a | Multisite gateway vector for 3' tagging with rab34a |
| 109694 | p3E-rab34b | Multisite gateway vector for 3' tagging with rab34b |
| 109696 | p3E-rab35b | Multisite gateway vector for 3' tagging with rab35b |
| 109697 | p3E-rab36 | Multisite gateway vector for 3' tagging with rab36 |
| 109699 | p3E-rab38a | Multisite gateway vector for 3' tagging with rab38a |
| 109701 | p3E-rab39ba | Multisite gateway vector for 3' tagging with rab39ba |
| 109702 | p3E-rab39bb | Multisite gateway vector for 3' tagging with rab39bb |
| 109703 | p3E-rab3aa | Multisite gateway vector for 3' tagging with rab3aa |
| 109704 | p3E-rab3ab | Multisite gateway vector for 3' tagging with rab3ab |
| 109705 | p3E-rab3c | Multisite gateway vector for 3' tagging with rab3c |
| 109706 | p3E-rab3da | Multisite gateway vector for 3' tagging with rab3da |
| 109707 | p3E-rab3db | Multisite gateway vector for 3' tagging with rab3db |
| 109708 | p3E-rab40b | Multisite gateway vector for 3' tagging with rab40b |
| 109709 | p3E-rab40c | Multisite gateway vector for 3' tagging with rab40c |
| 109710 | p3E-rab42a | Multisite gateway vector for 3' tagging with rab42a |
| 109711 | p3E-rab42b | Multisite gateway vector for 3' tagging with rab42b |
| 109713 | p3E-rab44 | Multisite gateway vector for 3' tagging with rab44 |
| 109714 | p3E-rab4a | Multisite gateway vector for 3' tagging with rab4a |
| 109715 | p3E-rab4b | Multisite gateway vector for 3' tagging with rab4b |
| 109716 | p3E-rab5aa | Multisite gateway vector for 3' tagging with rab5aa |
| 109717 | p3E-rab5ab | Multisite gateway vector for 3' tagging with rab5ab |
| 109718 | p3E-rab5b | Multisite gateway vector for 3' tagging with rab5b |
| 109719 | p3E-rab5c | Multisite gateway vector for 3' tagging with rab5c |
| 109720 | p3E-rab6a | Multisite gateway vector for 3' tagging with rab6a |
| 109721 | p3E-rab6ba | Multisite gateway vector for 3' tagging with rab6ba |
| 109722 | p3E-rab6bb | Multisite gateway vector for 3' tagging with rab6bb |
| 109723 | p3E-rab7a | Multisite gateway vector for 3' tagging with rab7a |
| 109724 | p3E-rab7b | Multisite gateway vector for 3' tagging with rab7b |
| 109725 | p3E-rab8a | Multisite gateway vector for 3' tagging with rab8a |
| 109726 | p3E-rab8b | Multisite gateway vector for 3' tagging with rab8b |
| 109727 | p3E-rab9a | Multisite gateway vector for 3' tagging with rab9a |
| 109728 | p3E-rab9b | Multisite gateway vector for 3' tagging with rab9b |
| 109729 | pME-mKate2-NS | Multisite gateway vector for 5' tagging with mKate2 |
| 109731 | pDEST-actc1b-ccdb-mKate2 | Multisite gateway destination vector for muscle specific expression of constructs tagged at the 3' end with mKate2 |
| 109732 | pDEST-actc1b-mKate2-ccdb | Multisite gateway destination vector for muscle specific expression of constructs tagged at the 5' end with mKate2 |
| 109733 | p3E-rab6a-CA | Multisite gateway vector for 3' tagging with constitutively active rab6a |
| 109734 | p3E-rab23-DN | Multisite gateway vector for 3' tagging with dominant negative rab23 |
| 109735 | p3E-rab4a-DN | Multisite gateway vector for 3' tagging with dominant negative rab4a |
| 109736 | p3E-rab4a-CA | Multisite gateway vector for 3' tagging with constitutively active rab4a |
| 109737 | p3E-rab5c-DN | Multisite gateway vector for 3' tagging with dominant negative rab5c |
| 109738 | p3E-rab5c-CA | Multisite gateway vector for 3' tagging with constitutively active rab5c |
| 109745 | p3E-rab26-DN | Multisite gateway vector for 3' tagging with dominant negative rab26 |
| 109746 | p3E-rab26-CA | Multisite gateway vector for 3' tagging with constitutively active rab26 |
| 109748 | p3E-rab38a-CA | Multisite gateway vector for 3' tagging with constitutively active rab38a |
| 109750 | actb2-lifeact-mKate2 | Ubiquitous zebrafish expression of mKate2 fused to the actin binding lifeact sequence |
| 109751 | actc1b-actn2-mKate2 | Muscle specific zebrafish expression of mKate2 fused to alpha actinin 2 |
| 109753 | p3E-Rab19 | Multisite gateway vector for 3' tagging with Rab19 |
| 109754 | p3E-Rab27b | Multisite gateway vector for 3' tagging with Rab27b |
| 109755 | p3E-Rab31 | Multisite gateway vector for 3' tagging with Rab31 |
| 109757 | p3E-Rab38b | Multisite gateway vector for 3' tagging with Rab38b |
| 109758 | p3E-Rab38c | Multisite gateway vector for 3' tagging with Rab38c |
| 109759 | p3E-Rab41 | Muscle specific zebrafish expression of mKate2 fused to p3E-Rab41 |
| 109760 | actc1b-mKate2-Rab3b | Muscle specific zebrafish expression of mKate2 fused to Rab3b |
| 109761 | actc1b-mKate2-Rab19 | Muscle specific zebrafish expression of mKate2 fused to Rab19 |
| 109762 | actc1b-mKate2-Rab27b | Muscle specific zebrafish expression of mKate2 fused to Rab27b |
| 109763 | actc1b-mKate2-Rab31 | Muscle specific zebrafish expression of mKate2 fused to Rab31 |
| 109765 | actc1b-mKate2-Rab38b | Muscle specific zebrafish expression of mKate2 fused to Rab38b |
| 109766 | actc1b-mKate2-Rab38c | Muscle specific zebrafish expression of mKate2 fused to Rab38c |
| 109767 | actc1b-mKate2-Rab41 | Muscle specific zebrafish expression of mKate2 fused to Rab41 |
| 109768 | pT3TS_Transposase | Codon optimised transposase sequence in an optimal vector for in vitro transcription. Contains the T3 promoter and synthetic polyA tract. |
| 109769 | p3E-rev_U6_MCS | Multisite gateway vector for 3' expression of a guide RNA from the U6 promoter (cassette in the reverse orientation). Contains BseRI sites for insertion of gRNA sequence |
| 115549 | pME-Lifeact-mEos2-NS | Multisite gateway vector for 5' tagging with Lifeact-mEos2. Used to make 115563. Parton lab clone IDA |
| 115550 | p3E-APEX2 (GC rich) | Multisite gateway vector for 3' tagging with GC-rich APEX2. Used to make 115563. Parton lab clone HHS |
| 115563 | Lifeact-mEos2-APEX2 | Lifeact-mEos2-APEX2 fusion for PALM and CLEM. Parton lab clone IET |
| 118628 | SITS-EGFP-ccdb | Multisite gateway destination vector for 5' tagging with EGFP for cell free expression. Parton lab clone FEZ |
| 118629 | SITS-mCherry-ccdb | Multisite gateway destination vector for 5' tagging with mCherry for cell free expression. Parton lab clone FKZ |
| 118752 | SITS-ccdb-EGFP | Multisite gateway destination vector for 3' tagging with EGFP for cell free expression. Parton lab FEX |
| 118753 | SITS-ccdb-mCherry | Multisite gateway destination vector for 3' tagging with mCherry for cell free expression. Parton lab clone FFF |
| 123628 | actb2-SecAnxaV-mKate2-acry-EGFP | Ubiquitous zebrafish expression vector containing secreted human AnnexinV tagged to mKate2. Parton lab clone BDN. |
| 126748 | p5E-smyhc1 | Zebrafish slow myosin heavy chain promoter. Clone 667 |
| 126749 | actc1b-mKate2-P2A-APEX2-VHH[GFP] | Expression of GFP binding nanobody fused to APEX2 in zebrafish muscle. Parton lab clone DSJ |
| 140864 | pT7gRNA-ccdb | Backbone for making sgRNAs. |
| 140866 | pT7gRNA-ttn.1_C3 | sgRNA synthesis vector for ttn.1_C3 (zebrafish titin .1). |
| 140867 | pT7gRNA-smyhc1_977 | sgRNA synthesis vector for smyhc1_977 (zebrafish slow myosin heavy chain 1). |
| 140873 | nls-transposase-nls-pA in pT3TS-Dest | Vector for in vitro transcription of Tol2 nls-transposase-nls for zebrafish transgenesis using T3. |
| 140874 | pME-mScarlet-NS | Multisite Gateway middle entry vector for fusing mScarlet to the 5' end of an ORF. |
| 140875 | p3E-mScarlet | Multisite Gateway middle entry vector for fusing mScarlet to the 3' end of an ORF. |
| 140876 | pME-mScarlet-STOP | Multisite Gateway middle entry vector for expression of untagged mScarlet. |
| 140877 | p3E-EGFP | 126573: Multisite Gateway middle entry vector for fusing mScarlet to the 3' end of an ORF. |
| 140878 | pT3TS-Dest_R1-R3 | 166571: Multisite gateway destination vector for in vitro transcription of RNA using T3. Accepts one pME and one p3E entry clone. |
| 140879 | EGFP-Rab13 in pCSDEST2 | Expression of zebrafish GFP-tagged Rab13 in mammalian cells. |
| 140880 | EGFP-Rab8a in pCSDEST2 | Expression of zebrafish GFP-tagged Rab8a in mammalian cells. |
| 140881 | EGFP-Rab8b in pCSDEST2 | Expression of zebrafish GFP-tagged Rab8b in mammalian cells. |
| 140882 | EGFP-Rab33bb in pCSDEST2 | Expression of zebrafish GFP-tagged Rab33bb in mammalian cells. |
| 140883 | EGFP-Rab40b in pCSDEST2 | Expression of zebrafish GFP-tagged Rab40b in mammalian cells. |
| 140884 | EGFP-Rab26 in pCSDEST2 | Expression of zebrafish GFP-tagged Rab26 in mammalian cells. |
| 140885 | EGFP-Rab32b | Expression of zebrafish GFP-tagged Rab32b in mammalian cells. |
| 140886 | EGFP-Rab37 | Expression of zebrafish GFP-tagged Rab37 in mammalian cells. |
| 140887 | EGFP-Rab38a | Expression of zebrafish GFP-tagged Rab38a in mammalian cells. |
| 140888 | EGFP-Rab19 | Expression of zebrafish GFP-tagged Rab19 in mammalian cells. |
| 140890 | EGFP-CaaX(tH) in pCSDEST2 | Expression of GFP-CaaX (sequence from Hras) in mammalian cells. |
| 141067 | SITS-ccdb | Multisite gateway destination vector for cell free expression (takes two cassettes). Parton lab clone JBO |
| 176026 | pME-nCas9n-P2A-TagBFP2-pA | Multisite gateway vector for expression of nCas9n with blue reporter. Parton lab clone ENT |