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Efficient genome editing in pathogenic mycobacteria using Streptococcus thermophilus CRISPR1-Cas9.
Meijers AS, Troost R, Ummels R, Maaskant J, Speer A, Nejentsev S, Bitter W, Kuijl CP
Tuberculosis (Edinb). 2020 Sep;124:101983. doi: 10.1016/j.tube.2020.101983. Epub 2020 Aug 12. PubMed Article

Plasmids from Article

ID Plasmid Purpose
140993pCRISPRx-Sth1Cas9-L5Sth1Cas9, TetR, KanR, L5Int, attP to create frameshifts and gene deletion in Mycobacteria
140994pTdTomato-L5tdTomato, StrepR, L5int, attP to create RFP mycobacteria

Antibodies from Article