Preloading budding yeast with all-in-one CRISPR/Cas9 vectors for easy and high-efficient genome editing.
Degreif D, Kremenovic M, Geiger T, Bertl A
J Biol Methods. 2018 Sep 12;5(3):e98. doi: 10.14440/jbm.2018.254. eCollection 2018. PubMed Article
Degreif D, Kremenovic M, Geiger T, Bertl A
J Biol Methods. 2018 Sep 12;5(3):e98. doi: 10.14440/jbm.2018.254. eCollection 2018. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 141249 | pCAS9i | Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites. |
| 141250 | pCAS9c | Constitutive expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites. |
| 141251 | pCAS9id | Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites. |
| 141252 | pCAS9cd | Constitutive expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites. |